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Merck
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主要文件

N6019

Sigma-Aldrich

Neutralizing Buffer

for Northern and Southern blotting, powder

别名:

Neutralizing Buffer for Blotting Techniques

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1 L
$184.00
5 X 1 L
$681.00

$184.00


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选择尺寸

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1 L
$184.00
5 X 1 L
$681.00

About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

$184.00


请联系客服了解存货情况

一般說明

Sigma′s neutralization buffer is suitable for Southern blotting procedures [1] to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.

應用

Suitable for neutralizing the gel before transfer to membranes during Southern procedures.

成分

The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)

实验方案

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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