推荐产品
等級
for molecular biology
產品線
BioReagent
形狀
powder
技術
electrophoresis: suitable
pH值
6.8-7.2(reconstituted per directions)
溶解度
H2O: soluble, faintly yellow
異物活動
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
儲存溫度
room temp
一般說明
MOPS-EDTA-乙酸钠(MESA)是Northern印迹程序之前RNA电泳最常用的运行缓冲液。 对于最大值分离度,建议施加5 V/cm的电压。
應用
适合在制备甲醛-琼脂糖凝胶和RNA电泳相关的运行缓冲液时使用。
成分
1x MESA 缓冲液包含:
- 40 mM MOPS
- 10 mM乙酸钠
- 1 mM EDTA (pH 8.3)
重構
将瓶中的全部内容物溶解在最终体积为1L的分子生物学级水中。 这产生了10X MESA贮备液,可以根据需要进一步稀释。
相關產品
产品编号
说明
价格
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Molecular Cloning: A Laboratory Manual, 7-7 (1989)
Molecular techniques for detection of gene expression in neuroimmunology.
Methods in Neuroscience, 24, 41-41 (1995)
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Electrophoresis in agarose and acrylamide gels.
Methods in enzymology, 152, 61-87 (1987-01-01)
实验方案
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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