94060901

FTC-133 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 别名: FTC133 Cells
• UNSPSC代码: 41106514

属性

• 生物来源: human thyroid (lymph node metastasis)
• 描述: Human follicular thyroid carcinoma
• 表单: liquid
• 包装: tube of 5 μg 94060901-DNA-5UG; pkg of vial of cells 94060901-1VL
• 生长模式: Adherent
• 核型: Complex chromosomal changes
• 形态学: Polymorphic
• 产品: Expression of 5′ - Deiodinase Type I
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: metastasis

说明

细胞系来源

Human follicular thyroid carcinoma, lymph node metastasis

细胞系描述

FTC-133 was obtained from a lymph node metastasis of a follicular thyroid carcinoma not the primary tumour as originally reported from a 42-year-old male. The morphology differs from flat polygonal to spindle formed cells. They retained differentiated thyrocyte function and thyrocyte responsiveness to thyrotropin and local active growth factors. Thyroglobulin immunoreactivity could be shown in the cytoplasm and EGF receptor immunoreactivity could be visualised on the cell membranes. Complex chromosomal changes were detected as well as a p53 mutation. FTC-133 (Sigma catalogue number 94060901), FTC-236 (Sigma catalogue number 06030202) and FTC-238 (Sigma catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

应用

FTC-133 cell line has been used to study the function of human thyroid and development of thyroid cancer.[1][2]

包装

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 9.3
TPOX: 9
vWA: 15,18

培养基

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:8 to 1:12 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable