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Merck

71736

Sigma-Aldrich

十二烷基硫酸钠 溶液

BioUltra, for molecular biology, 10% in H2O

别名:

SDS, 月桂基硫酸盐 钠盐, 月桂基硫酸钠 溶液

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100 ML
$167.00
500 ML
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About This Item

线性分子式:
CH3(CH2)11OSO3Na
CAS号:
分子量:
288.38
Beilstein:
3599286
MDL號碼:
分類程式碼代碼:
12161902
eCl@ss:
39093306
PubChem物質ID:
NACRES:
NA.26

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等級

for molecular biology

品質等級

描述

anionic

產品線

BioUltra

形狀

liquid

分子量

288.38 g/mol

濃度

10% in H2O

技術

HPLC: suitable
protein quantification: suitable

雜質

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH值

5.0-6.5

密度

1.01 g/mL at 20 °C

負離子痕跡

chloride (Cl-): ≤50 mg/kg
phosphate (PO43-): ≤1 mg/kg

正離子痕跡

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

&lambda ;

neat

紫外吸收

λ: 260 nm Amax: 0.3
λ: 280 nm Amax: 0.2

SMILES 字串

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI 密鑰

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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應用

十二烷基硫酸钠溶液已用于:
  • 制备2x提取缓冲液[1]
  • 作为用于免疫沉淀的洗脱缓冲液中的组分[2]
  • SDS-聚丙烯酰胺凝胶电泳(PAGE)分析[3][4]

生化/生理作用

十二烷基硫酸钠(SDS)/十二烷基硫酸钠是一种离子去污剂,具有破坏生物膜的能力。它可以阻断核糖核酸酶(RNase)和脱氧核糖核酸酶(DNase)活性。SDS是几种试剂的主要成分之一,可用于核酸纯化。[5]它也被认为是口服制剂中的渗透性增强剂。[6]

其他說明

在RNA的大规模苯酚提取中,其可以在从生物材料中进行提取时促进蛋白质与核酸的解离[7]

也與該產品經常一起購買

产品编号
说明
价格

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Resilient Ribonucleases
RNA Methodologies (2010)
Construction of FWPV Chimaeric MVA
Buttigieg K R and Skinner M A
Bio-protocol, 5(1) (2015)
Dictyostelium cultivation, transfection, microscopy and fractionation
Hirst J, et al.
Bio-protocol, 5(11) (2015)
Porous nucleating agents for protein crystallization
Khurshid S, et al.
Nature Protocols, 9(7), 1621-1621 (2014)
D.M. Wallace
Methods in Enzymology, 125, 39-39 (1987)

实验方案

TRI试剂是一种单步RNA分离试剂,可同时分离DNA、RNA和蛋白质。本实验方案概述了样品制备方法,以及各种样品提取方法和故障排除方案。

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. I WOULD LIKE TO REQUEST THE QUALITY CONFIRMATION OF %CONCENTRATION, SINCE CONCENTRATION IS 10% IN WATER, THERE'S NO INFORMATION THAT WHAT'S % TYPE [%W/V OR %V/V] REF.PRODUCT CODE: 71736

    1 answer
    1. This product is 10% (w/w) solution.

      Helpful?

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