跳转至内容
Merck

63689

Sigma-Aldrich

2-巯基乙醇

BioUltra, for molecular biology, ≥99.0% (GC)

别名:

β-巯基乙醇, 2-羟乙基硫醇, BME, 硫代二甘醇

登录查看公司和协议定价

选择尺寸

25 ML
$98.00
100 ML
$184.00

$98.00


有货详情


获取大包装报价

选择尺寸

变更视图
25 ML
$98.00
100 ML
$184.00

About This Item

线性分子式:
HSCH2CH2OH
CAS号:
分子量:
78.13
Beilstein:
773648
EC號碼:
MDL號碼:
分類程式碼代碼:
12161504
PubChem物質ID:
NACRES:
NA.25

$98.00


有货详情


获取大包装报价

等級

for molecular biology

品質等級

蒸汽密度

2.69 (vs air)

蒸汽壓力

1 mmHg ( 20 °C)

產品線

BioUltra

化驗

≥99.0% (GC)

形狀

liquid

expl. lim.

18 %

反應適用性

reagent type: reductant

濃度

14.3 M (pure liquid)

技術

RNA extraction: suitable
protein extraction: suitable

雜質

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

折射率

n20/D 1.500 (lit.)
n20/D 1.501

pH值

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

溶解度

H2O: 0.5 M at 20 °C, clear, colorless

密度

1.114 g/mL at 25 °C (lit.)

正離子痕跡

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.05 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES 字串

OCCS

&lambda ;

0.5 M in H2O

紫外吸收

λ: 260 nm Amax: 1.5
λ: 280 nm Amax: 0.3

儲存溫度

2-8°C

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI 密鑰

DGVVWUTYPXICAM-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

應用

2-巯基乙醇已用于
  • 叶子样品蛋白提取缓冲液中。[1]
  • 作为细胞培养基的补充剂。[2]
  • RNA提取过程中。[3]
BME 适用于在聚丙烯酰胺凝胶电泳前还原蛋白质二硫键,通常用于 SDS-PAGE 的样品缓冲液,浓度为 5%。 切割分子间(亚基之间)二硫键允许蛋白质的亚基在 SDS-PAGE 上独立分离。 切割分子内(亚基内)二硫键可使亚基完全变性,从而使每个肽根据其链长迁移,而不受二级结构的影响。

其他說明

使用胍盐对RNA进行分离。其包括利用还原剂对分子内蛋白质二硫键进行破坏,以增强变性[4]

訊號詞

Danger

危險分類

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

標靶器官

Liver,Heart

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

165.2 °F - closed cup

閃點(°C)

74 °C - closed cup

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells.
Benitez JJ et al.
Lab on a chip, 12, 4848-4848 (2012)
The interplay between protein L-isoaspartyl methyltransferase activity and insulin-like signaling to extend lifespan in Caenorhabditis elegans.
Khare S et al.
PLoS ONE, 6, e20850-e20850 (2011)
Adam Healey et al.
Plant methods, 10, 21-21 (2014-07-24)
Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within their leaf tissue making genomic DNA extraction difficult. While many DNA extraction
Isolation of RNA using guanidinium salts.
R J MacDonald et al.
Methods in enzymology, 152, 219-227 (1987-01-01)
Katharina Rothe et al.
Blood, 123(23), 3622-3634 (2014-04-24)
Previous studies demonstrated that imatinib mesylate (IM) induces autophagy in chronic myeloid leukemia (CML) and that this process is critical to cell survival upon therapy. However, it is not known if the autophagic process differs at basal levels between CML

商品

Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.

实验方案

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. How do I know when 2-Mercaptoethanol expires

    1 answer
    1. This material is quite stable when stored and handled properly. One visual indication of degradation would be a color change to a darker yellow to brown. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

      Helpful?

Reviews

No rating value

Active Filters

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门