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Merck

I9271

Sigma-Aldrich

5-(碘乙酰胺基)荧光素

≥90% purity (HPLC), powder

别名:

5-IAF

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About This Item

经验公式(希尔记法):
C22H14INO6
CAS号:
分子量:
515.25
Beilstein:
6543244
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47

product name

5-(碘乙酰胺基)荧光素, ≥90% (HPLC)

品質等級

化驗

≥90% (HPLC)

形狀

powder

顏色

yellow to orange

溶解度

DMSO: 5 mg/mL

螢光

λex 492 nm; λem 515 nm (Mercaptoethanol adduct)
λex 493 nm; λem 522 nm (pH 9.2)

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

−20°C

SMILES 字串

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(NC(=O)CI)ccc45)c1

InChI

1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27)

InChI 密鑰

UATCLPJEZJKNHE-UHFFFAOYSA-N

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一般說明

5-(碘乙酰氨基)荧光素是一种硫醇反应性荧光素衍生物。

應用

5-(碘乙酰氨基)荧光素用于合成荧光标记的细胞器(细胞核和核基质)、蛋白质和肽,包括酶。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase.
Tyson PA
The Journal of Biological Chemistry, 264, 726-726 (1989)
Multisite phosphorylation of the Saccharomyces cerevisiae filamentous growth regulator Tec1 is required for its recognition by the E3 ubiquitin ligase adaptor Cdc4 and its subsequent destruction in vivo.
Bao MZ
Eukaryotic Cell, 9, 31-31 (2010)
Meng M Rowland et al.
Nucleic acids research, 42(14), 9295-9303 (2014-07-14)
The DNA backbone is often considered a track that allows long-range sliding of DNA repair enzymes in their search for rare damage sites in DNA. A proposed exemplar of DNA sliding is human 8-oxoguanine ((o)G) DNA glycosylase 1 (hOGG1), which
D Chhabra et al.
Biophysical journal, 89(3), 1902-1908 (2005-07-05)
Actin is the principal component of microfilaments. Its assembly/disassembly is essential for cell motility, cytokinesis, and a range of other functions. Recent evidence suggests that actin is present in the nucleus where it may be involved in the regulation of
Gábor Glatz et al.
The Journal of biological chemistry, 288(12), 8596-8609 (2013-02-06)
Mitogen-activated protein kinase (MAPK) activation depends on a linear binding motif found in all MAPK kinases (MKK). In addition, the PB1 (Phox and Bem1) domain of MKK5 is required for extracellular signal regulated kinase 5 (ERK5) activation. We present the

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