PPB005

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween^® 20, pHast Pack^™

• 别名: PBS + 0.05% TWEEN^® 20, PBS with 0.05% Tween 20, PBS-T, PBST
• UNSPSC代码: 12161700
• NACRES: NA.21

属性

• 产品名称: 磷酸盐缓冲剂，含0.05% TWEEN^® 20，pH 7.4, pHast Pack^™, powder
• 产品线: pHast Pack^™
• 表单: powder
• 储存条件: (Tightly closed. Dry)
• 杂质: ≤5 ppm Heavy Metals (lead)
• 颜色: white
• pH值（酸碱度）: 7.2—7.6 (25 °C)
• 溶解性: soluble, clear to hazy, colorless
• 痕量阳离子: Fe: <10 ppm
• 异质活性: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• 储存温度: room temp

说明

一般描述

Phosphate-buffered saline with 0.05% Tween^® 20 or PBST contains PBS buffer with a low-concentration of Tween detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

应用

PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2[1].

特点和优势

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

包装

Foil pouches

重悬

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

储存及稳定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

其他说明

For additional information on our range of Biochemicals, please complete this form.

法律信息

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable