This product is a 1 mg/mL solution of BSA in 0.15 M NaCl. There are no anticipated problems with further diluting in Tris-based or other aqueous buffer solutions for blocking purposes.
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生物来源
bovine
质量水平
表单
liquid
包含
0.05% sodium azide as preservative
包装
ampule of 1 mL
浓度
0.98—1.02 mg protein/mL
技术
ELISA: suitable
颜色
clear colorless
UniProt登记号
储存温度
2-8°C
基因信息
bovine ... ALB(280717)
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相关类别
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
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商品
Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.
实验方案
To standardize a procedure for the determination of protein by modified Lowry.
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
相关内容
蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
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Can bsa P0914-5AMP be diluted in tbs-t to make block buffer for electrophoresis?
1 answer-
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What is the shipping temperature?
1 answer-
The item is shipped at ambient temperature. A transit time of up to 2 weeks will not impact the quality of this material. Upon delivery, this product should be stored at 2-8°C.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do you have any information on the traceability to a recognized protein standard, such as NIST? Or is the concentration derived from accepted values of natural physical constants?
1 answer-
The concentration of each lot of the protein standard, Product P0914, is determined by UV measurements. Specifically, given a known extinction coefficient (E) and a measured absorbance (A), and assuming a standard pathlength of 1 cm, the concentration (c) can be determined by Beer's Law (c = A/E). The assay result is derived from an accepted value of a natural physical constant. Specifically, the E1% of 6.67 at approximately 279 nm is a well-accepted value, originally published in J. Am. Chem. Soc., 78, 3656-3660 (1956).
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