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Merck
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主要文件

P0914

Sigma-Aldrich

蛋白标准品

Micro Standard, liquid

别名:

牛血清白蛋白,蛋白标准品

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5 AMP
$71.80
10 AMP
$125.00

$71.80


预计发货时间2025年4月07日详情

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5 AMP
$71.80
10 AMP
$125.00

About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.27

$71.80


预计发货时间2025年4月07日详情

新价格,新优惠!

获取大包装报价

生物来源

bovine

质量水平

表单

liquid

包含

0.05% sodium azide as preservative

包装

ampule of 1 mL

浓度

0.98—1.02 mg protein/mL

技术

ELISA: suitable

颜色

clear colorless

UniProt登记号

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

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应用

用于所有蛋白质测定的浓缩溶液。
蛋白标准品已用作:

  • 通过二辛可宁酸测定法量化蛋白释放的标准品[1]
  • 通过Bradford测定法量化尿素的标准品[1]
  • 通过原生聚丙烯酰胺凝胶电泳法量化蛋白质的分子大小标记物[2]
所有蛋白质测定的蛋白质标准品。密封安瓿保证每次测定的浓度准确无误。

包装

密封安瓿瓶

其他说明

在 0.15M NaCl 中,每 ml 含 1mg 牛血清白蛋白

适用性

该产品通常用于通过二喹啉甲酸 (BCA) 法 测定蛋白含量

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.

实验方案

To standardize a procedure for the determination of protein by modified Lowry.

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

相关内容

蛋白定量方法、试剂和免疫测定技术可准确测定各类样品中的蛋白浓度。

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Questions

1–4 of 4 Questions  
  1. Can bsa P0914-5AMP be diluted in tbs-t to make block buffer for electrophoresis?

    1 answer
    1. This product is a 1 mg/mL solution of BSA in 0.15 M NaCl. There are no anticipated problems with further diluting in Tris-based or other aqueous buffer solutions for blocking purposes.

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  2. What is the shipping temperature?

    1 answer
    1. The item is shipped at ambient temperature. A transit time of up to 2 weeks will not impact the quality of this material. Upon delivery, this product should be stored at 2-8°C.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Do you have any information on the traceability to a recognized protein standard, such as NIST? Or is the concentration derived from accepted values of natural physical constants?

    1 answer
    1. The concentration of each lot of the protein standard, Product P0914, is determined by UV measurements. Specifically, given a known extinction coefficient (E) and a measured absorbance (A), and assuming a standard pathlength of 1 cm, the concentration (c) can be determined by Beer's Law (c = A/E). The assay result is derived from an accepted value of a natural physical constant. Specifically, the E1% of 6.67 at approximately 279 nm is a well-accepted value, originally published in J. Am. Chem. Soc., 78, 3656-3660 (1956).

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