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BCA1

Sigma-Aldrich

BCA(Bicinchoninic Acid)蛋白定量试剂盒

for 200-1000 μg/ml protein

别名:

BCA蛋白检测

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1 KIT
$682.00

$682.00

预计发货时间2025年2月03日详情

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1 KIT
$682.00

About This Item

分類程式碼代碼:
12352106
NACRES:
NA.32

$682.00

预计发货时间2025年2月03日详情

新价格，新优惠！

品質等級

200

用途

sufficient for ~500 assays

儲存溫度

2-8°C

應用

二喹啉甲酸试剂盒被用来估量唾液蛋白组样本，[1]人胚肾293细胞[2]和黄体细胞中的蛋白质。[3]

特點和優勢

  • 简单、灵敏的比色法蛋白质检测
  • 两种稳定试剂作为工作溶液
  • 相对于Lowry蛋白检测，速度更快，操作更轻松
  • 兼容多种离子型及非离子型去垢剂
  • 相对于Bradford染料结合检测，不同蛋白质间的差异更小
  • 可针对微孔板应用进行灵活调整

原則

蛋白质将碱性Cu(II)还原为Cu(I)具有浓度依赖性。二辛可宁酸是一种应用于Cu(I)的高度特异性显色试剂，可以形成在562 nm处具有最高吸光度的紫色复合物。吸光度与蛋白浓度成正比。这是用于蛋白测定的Folin-Ciocalteu试剂的替代方法。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

仅试剂盒组分

产品编号
说明
  • Bicinchoninic Acid Solution 1 L
  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL
  • Protein Standard Solution 5 x 1

應用

产品编号
说明
价格

象形圖

CorrosionEnvironment
GHS05,GHS09

訊號詞

Danger

危險聲明

H290,H318,H410

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000412486
0000412486
0000389196
0000389196
0000368046

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商品

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

相关内容

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Questions

1–3 of 3 Questions  

  1. Can the kit be used for measuring the protein content of marine bacteria grown in artificial sea water?

    1 answer

    1. This product has not been tested for use with marine bacteria in artificial sea water. However, the publication below used the Bicinchoninic acid method to measure total protein in planktonic marine bacteria, indicating that this kit may be suitable.
      Determination of Total Protein Content of Bacterial Cells by SYPRO Staining and Flow Cytometry
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC91485/

      Note that there are a number of potential interfering substances that may impact results. Please see the link below to review the product datasheet, including a list of interfering substances beginning on page 4.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/246/793/bca1pis-ms.pdf

      Helpful?

  2. Does this kit rely on naked-eye colorimetric detection or does it require a fluorescent microscope?

    1 answer

    1. The kit is based on colorimetric, not fluorometric, detection. The color intensity should be measured at A562nm

      Helpful?

  3. Can a standard OD plate reader be used for the BCA1-1KT Kit, which offers a choice of assay tubes and 96-well plates as mentioned in the technical description? Additionally, would the Test tubes, 13 × 100 mm, Catalog Number CLS980013 not be needed?

    1 answer

    1. If a standard ELISA plate reader capable of measuring absorbance in the 560nm region is being used, the 96-well format should be suitable. The test tubes are recommended for larger volume analysis with cuvettes, but if the 96-well format is used, they will not be needed for the samples. However, they are also being utilized to make the BSA protein standard curve dilutions. If there is an alternative method for making the serial standard protein dilutions that ensures thorough mixing and utilizes a low-binding surface to prevent protein absorption, such as borosilicate glass and/or polypropylene tubes, that will be suitable as well.

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