This product will not prevent mitosis. However, if verapamil is used during the experiment (included in the kit to help with probe retention), it can inhibit mitosis in a dose-dependent manner.
SCT120
BioTracker 488活细胞核绿色染料
BioTracker 488 Green Nuclear Dye
Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
别名:
Live cell imaging probe
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About This Item
UNSPSC代码:
12352207
NACRES:
NA.47
价格与库存信息目前不能提供
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技术
cell based assay: suitable
检测方法
fluorometric
运输
ambient
一般描述
细胞核是真核细胞中的一种包膜细胞器。核内含有细胞′的大部分遗传基因，以多条DNA线性长链形式与各种蛋白（如组蛋白）复合形成染色体。过去，细胞核的显微分析一直采用DAPI和Hoechst等DNA染料，但是这些染料需要固定细胞，不能对活细胞显色。
BioTracker 488绿色活细胞核染料是一种可穿过细胞膜的绿色荧光DNA染料，可特异性染色活细胞或固定细胞中的细胞核。染料具有优异的DNA特异性，无需洗涤操作，低毒性适合活细胞成像。本品同时提供1瓶维拉帕米，一种外排泵抑制剂，可促进某些细胞的探针保留和活细胞染色。
注意：BioTracker细胞核染料还会在DAPI通道发出蓝色荧光，可能不适合与蓝色探针一起进行多色成像。
光谱性质
激发： 500nm
发射：515nm
BioTracker 488绿色活细胞核染料是一种可穿过细胞膜的绿色荧光DNA染料，可特异性染色活细胞或固定细胞中的细胞核。染料具有优异的DNA特异性，无需洗涤操作，低毒性适合活细胞成像。本品同时提供1瓶维拉帕米，一种外排泵抑制剂，可促进某些细胞的探针保留和活细胞染色。
注意：BioTracker细胞核染料还会在DAPI通道发出蓝色荧光，可能不适合与蓝色探针一起进行多色成像。
光谱性质
激发： 500nm
发射：515nm
应用
活细胞荧光成像
研究子类别
活细胞染料
活细胞染料
研究类别
细胞成像
细胞成像
适合活细胞核成像的绿色染料，光稳定性强于DAPI和Hoechst 33342等传统的蓝色荧光染料。
组分
1) BioTracker 488活细胞核绿色染料，1管×50µL，1000X DMSO溶液，CS224594
2) 盐酸维拉帕米，1管×100µL，100mM DMSO溶液，CS224592
2) 盐酸维拉帕米，1管×100µL，100mM DMSO溶液，CS224592
质量
光谱性质
激发： 500nm
发射：515nm
激发： 500nm
发射：515nm
外形
液体
储存及稳定性
BioTracker 488绿色细胞核染料2-8ºC储存。避光保存。
注意：打开小瓶前，短暂离心小瓶以收集小瓶底部的内容物。
注意：打开小瓶前，短暂离心小瓶以收集小瓶底部的内容物。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
警示用语：
Warning
危险声明
危险分类
Acute Tox. 4 Oral - Aquatic Chronic 2
储存分类代码
10 - Combustible liquids
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Could the BioTracker 488 Green Nuclear Dye SCT120 prevent mitosis in 2D in vitro cultures?
1 answer-
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Does the fluorescence intensity increase by chromosome condensation similarly to DAPI or Hoechst?
1 answer-
This product has not been tested for an increase in fluorescence intensity with chromosome condensation.
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Is there any toxicity data available for extended (>48h) live cell staining?
1 answer-
The dye with verapamil showed no obvious toxicity in MCF-7 cells over the course of 72 hours. It may be different for different cell lines and different experimental conditions.
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Is it possible to use SCT120 BioTracker 488 Green Nuclear Dye and PKH26 Red Fluorescent Cell Linker for in vivo applications, and what is their stability duration?
1 answer-
The PKH series of dyes are designed for membrane labeling and can be used to track cells through several divisions. The intensity of the dye decreases by half with each cell division, so the duration of visibility will depend on the cell division rate of the specific cell type.
Regarding SCT120 nuclear dye, there is limited information about the viability of cells after staining or the photostability of the dye once the cells have been stained.
Currently, there is no available information on the compatibility or effects of using SCT120 in conjunction with PKH26GL for dual staining of cells.
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How can I optimize staining using SCT120?
1 answer-
The recommended protocol for using SCT120 is as follows:
1. Dilute the BioTracker™ Nuclear Dye from its 1000X stock solution to a working concentration of 1X in cell culture medium. To achieve this, one could add 1 µL of dye to 1 mL of culture medium. It is important to note that the optimal concentration of the probe may vary depending on the cell type. Optionally, verapamil can be included in the staining solution to enhance probe retention in live cells. The ideal concentration of verapamil will likely be different across cell types, and it is suggested to test concentrations ranging from 10-100 µM.
2. Discard the culture medium from the cells and replace it with the diluted BioTracker™ Nuclear Dye solution. Incubate the cells at 37°C for a minimum of 10 minutes.
To optimize staining, consider the following adjustments:
• Probe Dilution: Although the standard dilution is 1:1000, experimenting with other dilutions such as 1:500 or 1:750 could be beneficial. Observe the results and, if necessary, test additional dilutions to find the most effective concentration.
• Verapamil Concentration: The suggested range for verapamil is between 10 to 100 µM. Trial with two or three different dilutions within this spectrum may help to refine the staining process.
• Incubation Time: Initially, a 10-minute incubation is recommended. However, extending this period to 20, 40, or even 60 minutes at 37°C could improve staining results. If positive changes are noted, further experimentation with the duration may be warranted to optimize the staining signal.
It is vital to only change one variable at a time during these optimizations. Altering multiple factors simultaneously, such as the dye dilution, verapamil concentration, and incubation time, would make it difficult to identify which specific change led to any observed improvements. Additionally, checking cell viability before staining is advisable. Using Trypan Blue to determine cell viability might provide insights into the overall health and suitability of the cells for staining.
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Will SCT120, BioTracker 488 Green Nuclear Dye SCT work with the FITC channel?
Will SCT120, BioTracker 488 Green Nuclear Dye SCT work with the FITC channel?
1 answer-
Yes, for SCT120 we do recommend analyzing the cells using the FITC channel.
Please refer to the product's data sheet, found in the DOCUMENTATION section of the Product Detail Page: https://www.sigmaaldrich.com/product/mm/sct120#product-documentation
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Could you please provide the chemical structure and formula for BIOTRACKER 488 GREEN NUCLEAR DYE, if available?
1 answer-
This information is considered proprietary.
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Hi I was wondering if this stain would work in a 100% alcoholic liquid, or whether it requires protons to fluoresce?
1 answer-
The dye has not been validated for DNA binding under 100% alcoholic liquid. It does not require protons to fluoresce.
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