The product is supplied as a 200 X solution. The specific formulation is considered proprietary. The dye is added at a rate of 5 ul per mL of culture medium. The lipophilic carbocyanine dyes have been used to stain neuronal cells in culture for several weeks, and in vivo for up to a year. The dyes do not appreciably affect cell viability and do not readily transfer between cells with intact membranes which allows for use in cell migration and tracking studies of mixed cell populations. The stability of labeling varies based on cell types and the rate of membrane turnover or cell division. Please see the link below to review a product Certificate of Analysis which serves as the datasheet:
https://www.emdmillipore.com/US/en/product/BioTracker-490-Green-Cytoplasmic-Membrane-Dye,MM_NF-SCT106#documentation
价格与库存信息目前不能提供
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技術
cell based assay: suitable
檢測方法
fluorometric
運輸包裝
ambient
一般說明
Lipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 484nm
Emission: 501nm
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 484nm
Emission: 501nm
應用
Live cell fluorescent imaging
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
品質
Spectral Properties
Absorbance: 484nm
Emission: 501nm
Absorbance: 484nm
Emission: 501nm
外觀
Liquid
儲存和穩定性
Store BioTracker 490 Green Cytoplasmic Membrane Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Flam. Liq. 2
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
55.4 °F
閃點（°C）
13 °C
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I am already using the BioTracker NIR750 and I am very satisfied. For this product I don't see the Data Sheet. At which concentration is it delivered? Which is the optimal final concentration to use? For how many round of cell division can be detected?
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