The R&D team confirmed that both the capture and detection antibodies are polyclonal goat anti-human Granulysin. The immunogen is recombinant Human Granulysin (aa23-145). The antibodies have not been tested for reactivity to different isoforms of Granulysin.
推荐产品
种属反应性
human
制造商/商品名称
Milliplex®
技术
multiplexing: suitable
检测方法
fluorometric (Luminex® xMAP®)
储存温度
2-8°C
一般描述
Immune checkpoint proteins are regulators of immune activation and play a key role in maintaining immune homeostasis, preventing autoimmunity, and are involved in cancer immune escape. These immune system molecules demonstrate the ability to act both as agonists (co-stimulatory checkpoint molecules) or antagonists (co-inhibitory checkpoint molecules) of immune response. Immune checkpoint inhibitor drugs targeting inhibitory immune checkpoint proteins, such as CTLA-4 and PD-L1, have demonstrated efficacy in cancer immunotherapy by improving the antitumor immune response and saving lives in the treatment of many malignancies. Immune checkpoint proteins may express on immune cells and/or tumor cells as activating receptors or inhibitory receptors. Many immune checkpoint proteins are also expressed as soluble forms in circulation and in the tumor and tumor microenvironment and are putative biomarkers for immunotherapy in various diseases including cancer.MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 2 is a 31-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture samples: 4-1BBL/TNFSF9, 5′-NT/CD73 (5′-nucleotidase), APRIL (TNFSF13), Arginase-1, B7-H2/ICOSL (Inducible T cell costimulator ligand), B7-H3/CD276, B7-H4/VTCN1 (V-set domain-containing T-cell activation inhibitor 1), B7-H5/VISTA (V-set immunoregulatory receptor), B7-H6 (Natural killer cell cytotoxicity receptor 3 ligand), BAFF/BLyS (TNFSF13B), CD25/IL-2Rα (Interleukin-2 receptor alpha), CD30/TNFRSF8, CD40L (CD40 ligand), CD137/4-1BB (TNFRSF9), CD226/DNAM-1 (DNAX accessory molecule 1), E-Cadherin, FGL1/Hepassocin (Fibrinogen-like protein 1), Galectin-1 (Gal-1), Galectin-3 (Gal-3), Granulysin, Granzyme B, IDO1 (Indoleamine 2,3-dioxygenase 1), MICA (MHC class I polypeptide-related sequence A), MICB (MHC class I polypeptide-related sequence B), Nectin-2 (PVRL2, CD112), Nectin-4 (PVRL4), OX40/CD134 (TNFRSF4), Perforin, PVR/CD155 (Poliovirus receptor cell adhesion molecule), Siglec-7 (Sialic acid-binding Ig-like lectin 7, CD328), Siglec-9 (Sialic acid-binding Ig-like lectin 9, CD329).
特异性
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
应用
The Human Immuno-Oncology Checkpoint Protein Panel 2 bead-based multiplex panel, using the Luminex® xMAP technology, enables the simultaneous analysis of 31 immune checkpoint proteins in human serum, plasma and cell culture samples.This is an overnight incubation assay (16-18 hours at 2-8°C).A maximum of 25 μL per well 1:2 diluted serum or plasma can be used. Tissue culture or other media may be run neat or appropriately diluted in control medium. Note: Best results using cell/tissue lysate samples may require diluting in assay buffer while testing similarly diluted lysis buffer for matrix effects.CD40 Ligand;4-1BBL/TNFSF9;Arginase-1;B7-H2/ICOSL;B7-H3/CD276;5′-NT/CD73;B7-H4/VTCN1;APRIL/TNFSF13;B7-H5/VISTA;CD25/IL-2Rα;B7-H6;CD137/4-1BB;Granzyme B;CD226/DNAM-1;CD30/TNFRSF8;E-Cadherin;FGL1/Hepassocin;Galectin-1 (GAL1);Galectin-3;Granulysin;IDO1;MICA;MICB;Nectin-2;BAFF/Blys;Nectin-4;OX40/CD134;PVR/CD155;Siglec-7;Siglec-9;Perforin
特点和优势
Design your multiplex kit by choosing available analytes within this panel.
包装
96-well plate
储存及稳定性
Recommended storage for kit components is 2 - 8°C.
法律信息
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
靶器官
Respiratory Tract
储存分类代码
10 - Combustible liquids
相关内容
Research evaluating immune checkpoints may help identify those likely to benefit from PD-1/PD-L1 immunotherapy and reveal new immunotherapy targets for future investigation. See how the MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 2 was used to multiplex immune checkpoint molecules for NSCLC research.
评估免疫检查点有助于明确在PD-1/PD-L1免疫疗法中获益的患者，并为后续研究揭示新的免疫治疗靶点。了解如何使用MILLIPLEX® Human Immuno-Oncology Checkpoint Protein Panel 2进行免疫检查点分子的多重检测评估来研究NSCLC疗法。
Cancer biomarker detection through MILLIPLEX® multiplex cancer assays can accelerate studies of tumorigenic processes helping advance cancer research.
Discover the benefits of MILLIPLEX® multiplex assays, based on Luminex® xMAP® multiplex assay technology, that provide consistent, high-quality results and see how these multiplex biomarker Luminex® assays are being used to advance research.
-
What is the specificity of the Granulysin antibody pair in the Milliplex Human Immuno-oncology Checkpoint Protein Panel?
1 answer-
Helpful?
-
-
How many samples can I analyze with one multiplex kit including all controls and assuming that I will have 5 different time points were I will need to analyze samples immediately?
1 answer-
The end-user can analyze 38 samples in duplicates.
Helpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
