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Merck
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CC176

Sigma-Aldrich

Skin dECM Gel Hydrogel Kit

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75
价格与库存信息目前不能提供

生物来源

Porcine

包装

pkg of 1 kit

制造商/商品名称

Sigma-Aldrich

生长模式

N/A

技术

cell culture | mammalian: suitable

运输

ambient

储存温度

2-8°C

一般描述

The cellular microenvironment directly and indirectly effects cellular behavior via both biophysical and biochemical routes. The microenvironment is composed of extracellular matrix proteins (ECM), other surrounding cells, cytokines, growth factors, hormones, and other bioactive agents and nano/microscale mechanical properties such as physical tension/stiffness. Decellularization is the process used in biomedical engineering to isolate the native extracellular matrix (ECM) of a tissue from its inhabiting cells, leaving an ECM scaffold of the original tissue, which can be used in cell culture and tissue regeneration. Compared to other tumor derived basement membrane extracts (Matrigel@), these dECM hydrogel scaffolds can provide a more physiological environment with increased growth rates for cells without the use of any exogenous growth factors. Our tissue-specific dECM Gel Hydrogel Kits are a series of native decellularized extracellular matrix (ECM) protein solutions derived from a variety of non-diseased porcine organs (bone, heart, liver, kidney, intestine, skin and lung). These hydrogels can be used as traditional 2D ECM coatings or as hydrogels to encapsulate cells for 3D cell culture applications.

应用

pH: 2.0-3.0Gelation: Forms GelBiocompatibility: 90% Cell ViabilitySterility: No Fungi or Bacteria DetectedMycoplasma: NegativePRRS, PRV: Negative

特点和优势

Skin decellularized ECM scaffold for traditional 2D and 3D cell culture applications.

储存及稳定性

Store all components of the dECM Gel Hydrogel Kit at 2-8°C (do not freeze).

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

Corrosion
GHS05

警示用语：

Warning

危险声明

H290

预防措施声明

P234 - P390

危险分类

Met. Corr. 1

储存分类代码

8B - Non-combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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商品

dECM hydrogels 3D culture

Comparison of decellularized extracellular matrix (dECM) hydrogels to Matrigel® matrices for predictive 3D cell culture models

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

3D细胞培养水凝胶

水凝胶是最常用的 3D 细胞培养系统。了解关于该技术的更多信息（什么是水凝胶？如何使用水凝胶？）

Questions

1–2 of 2 Questions  

  1. Does pH of the final hydrogel after adding component A, component B and cell suspension is 2.0 - 3.0? If yes, what is the best solution to adjust pH to 6.8 -7.4?

    1 answer

    1. Upon addition of Component A and Component B, the final pH should be approximately 7.4.

      Helpful?

  2. How much material is one Kit?

    1 answer

    1. The components of this kit are listed on the Product Datasheet, which can be found in the 'DOCUMENTATION' section under 'More Documents':
      Skin ECM Solution (CS226373): Qt. 2 (0.3 mL)
      Component A (CS226431): Qt. 1 (1 mL)
      Component B (CS226430): Qt. 1 (1 mL)

      Helpful?

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