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E0282

Sigma-Aldrich

MaxGel™ ECM

mixture, liquid

别名:

Human Basement Membrane Extract (BME)

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1 ML
$254.00

$254.00

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1 ML
$254.00

About This Item

分類程式碼代碼:
12352207
eCl@ss:
32190102
NACRES:
NA.71

$254.00

请联系客服了解存货情况
获取大包装报价

應用

MaxGel™ ECM被用于以下应用：
  • 细胞外基质（ECM）蛋白结合测定[1][2]
  • 细胞侵袭测定[3]
  • 细胞培养[4]
该产品提供了立体培养环境，可以促进细胞的生长和迁移，已被证实可以支持多种类型细胞的增殖，包括人胚胎干细胞、神经干细胞、神经细胞、神经胶质细胞、星形胶质细胞、成纤维细胞、肝实质细胞及角质形成细胞等。

成分

MaxGel ECM 含有多种人细胞外基质成分，包括胶原蛋白、层粘蛋白、纤连蛋白、腱生蛋白、弹性蛋白以及大量的蛋白聚糖及粘多糖等。这种基底膜提取物可以高效地重现上皮细胞与间质在皮肤发育及器官型细胞培养过程中的协同作用。

注意

在-70℃温度下，MaxGel可以至少稳定保存一年。

法律資訊

MaxGel is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCQ2147
SLCN0499
SLCJ9762
SLCK8873
SLCM8909

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商品

MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications

Human cell derived basement membrane extract (BME) hydrogel for 3D cell culture of primary cell, stem cell and xenograph cancer models.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

3D 细胞培养工具和技术

3D细胞培养概述。了解2D与3D细胞培养的对比结果，3D细胞培养的优势以及可用于开发3D细胞模型的技术

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

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Questions

1–7 of 7 Questions  

  1. How do I handle Product E0282, MaxGel™ ECM?

    1 answer

    1. Remove the product from the freezer and thaw on ice just prior to use. Keep the product cold until coating.

      Please see the link below to review the product datasheet which includes the coating protocol:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/243/108/e0282dat.pdf

      Helpful?

  2. is the Product E0282, MaxGel™ ECM from normal tissue or tumor tissue?

    1 answer

    1. This product is derived from the basement membranes of epithelial cells and fibroblasts. These are not strictly tumor cell lines as the normal genotype and phenotype are retained.

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  3. Can this product also be used for cell differentiation?

    1 answer

    1. The impact of extracellular matrix on cell differentiation can vary from cell type to cell type. This product is derived from human basement membrane extract that includes several components, many of which are not defined. Cell differentiation may be induced by the structural environment or other components present within the matrix. The end user would have to determine the suitability of the product based on the cell or tissue type of interest.

      Please refer to the literature for additional information. The following link may be of interest:
      https://biosignaling.biomedcentral.com/articles/10.1186/s12964-023-01252-8#:~:text=Several%20studies%20have%20demonstrated%20that,a%20certain%20niche%20%5B46%5D.

      Please see the link below to review the product datasheet for this item:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/243/108/e0282dat.pdf

      Helpful?

  4. How is Product E0282, MaxGel™ ECM, supplied?

    1 answer

    1. E0282 is supplied at a concentration of 1 mg/ml in DMEM.

      Helpful?

  5. Will Product E0282, MaxGel™ ECM, work for adherent cells and suspension cells?

    1 answer

    1. Yes, this product works for both cell types.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is an appropriate working concentration for Product E0282, MaxGel™ ECM?

    1 answer

    1. MaxGel™ works best undiluted or even diluted 1:2-1:4.  For a thin coat plating method, this material can be diluted as high as 1:100.

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