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Merck
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主要文件

14-392

Millipore

绿色荧光蛋白

别名:

GFP

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300 μG
$651.00

$651.00

预计发货时间2025年5月02日详情

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300 μG
$651.00

About This Item

UNSPSC代码:
12352200
eCl@ss:
32160405
NACRES:
NA.32

$651.00

预计发货时间2025年5月02日详情

重组

expressed in E. coli

质量水平

100

方案

>70%

表单

liquid

分子量

Mw ~28 kDa

包装

pkg of 300 μg

制造商/商品名称

Upstate®

技术

western blot: suitable

GenBank登记号

AAA27722

UniProt登记号

P42212

运输

dry ice

一般描述

对应于全长绿色荧光蛋白的N末端带有HIS标签的融合蛋白，在大肠埃希菌中表达。绿色荧光蛋白(GFP)是维多利亚多管发光水母(Aequorea Victoria)中的一种细胞质蛋白。[1]

生化/生理作用

绿色荧光蛋白(GFP)可以通过遗传改造，跟许多种蛋白形成融合蛋白，同时保持目标蛋白的活性和GFP的荧光。GFP可用作基因表达的报告基因，也可作为活细胞蛋白质定位研究的融合标签。[1]还作为光学和显微探针，分析各种系统的生物功能。[2]GFP非常稳定，适合作为体外研究工具使用。[1]

质量

常规评估为蛋白凝胶和蛋白质免疫印迹的标准品，用于校准荧光计以及显微注射到细胞和组织中。

外形

含20%甘油的PBS。

储存及稳定性

在-20°C下保存1年

法律信息

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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Elisa M York et al.
Biomedical optics express, 10(9), 4381-4394 (2019-10-01)
Autofluorescence of endogenous molecules can provide valuable insights in both basic research and clinical applications. One such technique is fluorescence lifetime imaging (FLIM) of NAD(P)H, which serves as a correlate of glycolysis and electron transport chain rates in metabolically active
J L Casey et al.
Protein engineering, 13(6), 445-452 (2000-07-06)
We produced a fluorescent antibody as a single recombinant protein in Escherichia coli by fusing a red-shifted mutant of green fluorescent protein (EGFP) to a single-chain antibody variable fragment (scFv) specific for hepatitis B surface antigen (HepBsAg). GFP is a
Vincent Jung et al.
Proteomics, 22(9), e2100031-e2100031 (2021-12-28)
Biolayer interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work, we combine BLI interaction measurement with mass spectrometry in order to
Dominique C S Evans et al.
Microbial cell (Graz, Austria), 10(7), 145-156 (2023-07-03)
Staphylococcus aureus is a major human pathogen that utilises many surface-associated and secreted proteins to form biofilms and cause disease. However, our understanding of these processes is limited by challenges of using fluorescent protein reporters in their native environment, because
Sonit Kumar Gogoi et al.
Langmuir : the ACS journal of surfaces and colloids, 22(22), 9322-9328 (2006-10-18)
Recombinant Escherichia coli (E. coli) bacteria expressing green fluorescent protein (GFP) was used as a model system to investigate the antimicrobial activities of Ag nanoparticles (NPs). A convenient in situ method of Ag NP synthesis using sodium borohydride, in the
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Questions

  1. このGFPはwild typeのGFPでしょうか。それともEGFPなど他の種類のGFPでしょうか。

    1 answer

    1. eGFP stands for enhanced green fluorescent protein, a red-shifted mutant of GFP, which folds more efficiently and fluoresces more intensely than wild-type GFP. This product corresponds to the green fluorescent protein (GFP) isolated from the jellyfish, Aequorea victoria.
      Please also see: https://www.ncbi.nlm.nih.gov/protein/AAA27722 and https://www.uniprot.org/uniprot/P42212.

      GFP absorbs blue light (maximum 395 nm, smaller absorbance peak at 470 nm) and emits green light (maximum 507-510 nm, shoulder at 545 nm) (PubMed:8137953, PubMed:9154981). The exact value of the emission maximum depends on the environment of the chromophore (PubMed:10220315).

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