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Merck
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21877

Sigma-Aldrich

5(6)-羧基荧光素

suitable for fluorescence, BioReagent, ≥95% (HPLC)

别名:

5(6)-FAM, 5,6-CF

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About This Item

经验公式（希尔记法）:
C21H12O7
CAS号:
72088-94-9
分子量:
376.32
EC號碼:
276-331-1
MDL號碼:
MFCD00151081
分類程式碼代碼:
12171500
PubChem物質ID:
329752213
NACRES:
NA.32

產品線

BioReagent

品質等級

200

化驗

≥95% (HPLC)

形狀

powder

mp

275 °C (dec.) (lit.)

螢光

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0

適合性

suitable for fluorescence

SMILES 字串

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35.OC(=O)c6ccc7C(=O)OC8(c9ccc(O)cc9Oc%10cc(O)ccc8%10)c7c6

InChI

1S/2C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21;22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h2*1-9,22-23H,(H,24,25)

InChI 密鑰

BPVHBBXCESDRKW-UHFFFAOYSA-N

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相关类别

一般說明

5(6)-羧基荧光素，也称为5(6)-FAM，是一种荧光示踪剂，可作为5-羧基荧光素和6-羧基荧光素异构体的混合物。5(6)-羧基荧光素的荧光是由它的杂蒽部分决定的。 5(6)-羧基荧光素有两个特性:
  • 它有两个波长的最大吸光度（465 和 490nm）。
  • 它的荧光发射在 515nm处（最大）随 pH 值的变化而增大，生理范围为 6-7.4。

應用

5(6)-羧基荧光素在体内用于肿瘤细胞和正常细胞的表征。它标记通过n -羟基琥珀酰亚胺（NHS）酯介导衍生化衍生的蛋白质。 5(6)-羧基荧光素适用于检测细胞活力，同时开发体外驱虫药试验。

其他說明

细胞内pH探针

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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S Mordon et al.
Journal of photochemistry and photobiology. B, Biology, 13(3-4), 307-314 (1992-05-15)
The pH of the interstitial fluid of malignant tumours tends to be lower than that of normal tissue and is depressed by glucose administration. This study aimed to evaluate the effectiveness of dual-wavelength fluorometry using a pH-dependent indicator (5,6-carboxyfluorescein: 5,6-CF)
Charlemagne Gnoula et al.
Talanta, 71(5), 1886-1892 (2007-03-30)
A new in vitro assay for anthelmintic activity using Caenorhabditis elegans is based on the ability of 5(6)-carboxyfluorescein diacetate (CFDA) to indicate the worm's viability. It is shown for the first time that the treatment of a suspension of worms
Jagpreet S Nanda et al.
Methods in enzymology, 536, 87-94 (2014-01-16)
N-hydroxysuccinimde (NHS) ester-mediated derivitization involves the reaction of this amine-reactive group with the primary amines of a protein or a biomolecule. Using NHS chemistry allows one to conjugate various fluorescent probes, biotin, and cross-linkers to primary amines. For example, we
M L Graber et al.
Analytical biochemistry, 156(1), 202-212 (1986-07-01)
We evaluated four different fluoroprobes to determine their capabilities and limitations in measuring intracellular pH by the fluorescent indicator technique. In vitro, carboxyfluorescein, dimethylcarboxyfluorescein, biscarboxyethyl carboxyfluorescein, and 4-methylumbelliferone (4MU) all showed comparably intense fluorescence and excellent pH sensitivity near their
Jorien Claes et al.
Blood, 124(10), 1669-1676 (2014-06-22)
Adhesion of Staphylococcus aureus to blood vessels under shear stress requires von Willebrand factor (VWF). Several bacterial factors have been proposed to interact with VWF, including VWF-binding protein (vWbp), a secreted coagulase that activates the host's prothrombin to generate fibrin.
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