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126741

Sigma-Aldrich

Alcalase® Enzyme, Bacillus licheniformis

别名:

Protease from Bacillus licheniformis, subtilisin A

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About This Item

EC 号:
3.4.21.14 (BRENDA, IUBMB)
UNSPSC代码:
12352202
NACRES:
NA.56
价格与库存信息目前不能提供

生物来源

Bacillus licheniformis

质量水平

100

表单

liquid

比活

≥0.75 Anson units/mL

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

技术

activity assay: suitable

颜色

dark brown

应用

life science and biopharma

运输

ambient

储存温度

2-8°C

一般描述

Alcalase® is a serine endopeptidase that consists primarily of subtilisin A.[1]

应用

Alcalase® Enzyme, Bacillus licheniformis has been used in the proteolytic digestion and extraction of polysaccharides in various sponge species.[2] It has also been used as an endoprotease enzyme in enzymatic hydrolysis for the production of fish protein hydrolysate.[3]

生化/生理作用

Alcalase® is suitable for the hydrolysis of proteins with an optimal pH of 7.0-8.5. It is used as an additive in detergent, dehairing, and bating leather. Alcalase is also used in meat tenderizing, cheese flavor development, baked manufacture, and improving the digestibility of animal feeds. Commercial Alcalase® comprises a liquid enzymatic preparation containing 50% glycerol, 41% water, and 9% protease extract from Bacillus licheniformis.[1]

警告

Toxicity: Harmful (C)

单位定义

One unit is defined as the amount of enzyme that will release 1.0 µmol L-tyrosine from hemoglobin per min at 25°C, pH 7.5.

重悬

Please refer to the vial label for lot-specific density.

法律信息

Alcalase is a registered trademark of Novozymes Corp.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Health hazardCorrosion
GHS08,GHS05

警示用语：

Danger

危险声明

H318,H334,H412

危险分类

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Veymar G Tacias-Pascacio et al.
International journal of biological macromolecules, 165(Pt B), 2143-2196 (2020-10-23)
This review aims to cover the uses of the commercially available protease Alcalase in the production of biologically active peptides since 2010. Immobilization of Alcalase has also been reviewed, as immobilization of the enzyme may improve the final reaction design
Monitoring of sulfated polysaccharide content in marine sponges by Raman spectroscopy
Maia LF, et al.
Vibrational Spectroscopy, 87, 149-156 (2016)
Fish protein hydrolysate production by acid and enzymatic hydrolysis
Wisuthiphaet N, et al.
Applied Science and Engineering Progress, 9(4) (2016)

Questions

1–2 of 2 Questions  

  1. What is the density or the concentration of the enzyme because I can't find it in the FDS ?

    1 answer

    1. Product density and activity are reported in the batch-specific Certificate of Analysis (CofA). The specifications are as follows: density ≥ 1.000 g/ml and activity ≥ 0.750 U/ml.

      Helpful?

  2. Can I dilute the enzyme in distilled/deionized water or should I use any specific buffer for dilution?

    1 answer

    1. Double distilled water can be used to further dilute product 126741.

      Helpful?

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