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Merck
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文件

07-1364

Sigma-Aldrich

抗-ECT2抗体

from rabbit, purified by affinity chromatography

别名:

epithelial cell transforming sequence 2 oncogene, Epithelial cell-transforming sequence 2 oncogene, protein ECT2

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

pig, human

物種活性(以同源性預測)

porcine (based on 100% sequence homology), rabbit (based on 100% sequence homology), monkey (based on 100% sequence homology), opossum (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)

技術

immunocytochemistry: suitable
western blot: suitable

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... ECT2(1894)

一般說明

上皮细胞转化序列2癌基因(ECT2)是鸟嘌呤核苷酸交换因子或GEF,属于Rho GEF的Dbl家族。ECT2由N端内的2个BRCT域,一个DBL同源域和一个PH域组成。研究表明,ECT2定位于细胞皮层和中央纺锤体,并且它是细胞增生过程中卵裂沟形成的关键部分。已显示仅包含N末端结构域的裂解的ECT2的过表达会引起多核化。作为GEF,已显示它与Rac蛋白,RhoA和RhoC特异性结合。

特異性

该抗体可识别
C端的ECT2。

免疫原

KLH缀合的线性肽,对应于人ECT2的C末端附近区域。
表位:C端

應用

抗ECT2抗体是针对ECT2 的抗体,可用于WB & IC。
研究子类别
G-蛋白
研究类别
信号传导
蛋白质印迹法(Snap i.d.)分析:5 µg/mL先前批次在A549细胞裂解液中检测到ECT2。
免疫细胞化学分析: 先前批次已被独立实验室用于Heya8细胞。(Der Channing,北卡罗来纳大学教堂山分校,NC,channing_der@med.unc.edu)

品質

已通过蛋白质印迹对A549细胞裂解液进行了评估。
蛋白质印迹分析:1 µg/mL该抗体可在10 µg的A549细胞裂解液中检测到ECT2。

標靶描述

可观察到约105 kDa的条带

外觀

亲和纯化
在含 0.1 M Tris-甘氨酸(pH 7.4),150 mM NaCl 和 0.05% 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。

儲存和穩定性

自收到之日起,在2-8°C条件下可稳定保存1年。

分析報告

对照
A549细胞裂解液

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Translational oncology, 11(3), 700-714 (2018-04-10)
Pancreatic cancer is the fourth leading cause for cancer-related death, and early diagnosis is one key to improve the survival rate of this disease. Molecular biomarkers are an important method for diagnostic use in pancreatic cancer. We used data from
Ming Yi et al.
Experimental hematology & oncology, 11(1), 107-107 (2022-12-27)
Breast cancer is the most common malignancy and the second leading cause of cancer-related death in women. Recent studies have indicated that aberrant activation of Rho GTPases relates to the malignant properties of breast cancer cells. As the guanine nucleotide
Xianguang Bai et al.
Cancer management and research, 10, 4039-4050 (2018-10-16)
Epithelial cell transforming sequence 2 (ECT2) is a guanine nucleotide exchange factor, which is involved in cell division regulation and cell cycle modulation. Recent evidence indicates that ECT2 is overexpressed in many human cancers. However, the exact prognostic value of
Dao-Yin Gong et al.
Oncology letters, 20(4), 54-54 (2020-08-14)
Gastric cancer remains the third leading cause of cancer-associated mortality worldwide. The identification of prognostic indicators that are associated with clinical characteristics is urgently required. The aim of the present study was to determine the involvement of epithelial cell transforming

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