Dye content is determined by back calculation from the carbon or nitrogen content, while purity is established by HPLC. The dye content is 60%. The impurities are not identified by either dye content or by methods such as HPLC, and no testing for impurities is conducted in this case. Both dye content and dye purity are determined for this product, with the Specification sheet listing minimum specifications for all lots, and individual Certificates of Analysis reporting lot-specific results. Water content is not measured, and the product is not considered hygroscopic.
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25 G
$247.00
100 G
$789.00
About This Item
线性分子式:
C34H24N6O14S4Na4
CAS号:
分子量:
960.81
顏色索引編號:
23850
Beilstein:
4360496
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
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品質等級
化驗
≥80% (HPLC)
形狀
powder
成份
Dye content, 60%
技術
microbe id | staining: suitable
顏色
dark green to brown, or Brown-Black
mp
>300 °C (lit.)
溶解度
H2O: 1 mg/mL
λmax
607 nm
&epsilon ；(消光係數)
≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol
應用
diagnostic assay manufacturing
hematology
histology
儲存溫度
room temp
SMILES 字串
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI 密鑰
GLNADSQYFUSGOU-UHFFFAOYSA-J
基因資訊
human ... PTPN1(5770) , PTPRF(5792)
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一般說明
台盼蓝（C.l.23850）是一种大的四磺化阴离子亲水染料。它也被称为二胺蓝 3B。
生化／生理作用
台盼蓝被大多数活细胞阻挡，但可被带入吞噬细胞和某些其他细胞。
该染料可根据′细胞吸收或排除染料的能力来区分死细胞和活细胞。活细胞应具有完整的细胞膜，因此将这种染料排除在外，并且细胞质是透明的。另一方面，没有活力的细胞显示出蓝色的细胞质。
该染料可根据′细胞吸收或排除染料的能力来区分死细胞和活细胞。活细胞应具有完整的细胞膜，因此将这种染料排除在外，并且细胞质是透明的。另一方面，没有活力的细胞显示出蓝色的细胞质。
台盼蓝通常用于各种细胞类型的活性测试，包括冷冻精子和接触杀真菌药物的主动脉肌细胞等。它也在Van Gieson手术中用作胶原蛋白染色。对于淀粉样蛋白染色，台盼蓝被认为是Puchtler-Bennhold染色中刚果红的蓝色替代品。 它适用于细胞活力测定：
- 通过用 0.2% 台盼蓝溶液染色来确定活细胞和坏死细胞的数量。
- 对植物组织进行染色以观察细胞死亡。
訊號詞
Danger
危險聲明
危險分類
Carc. 1B
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 3
個人防護裝備
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Are the descriptions of Product 302643 as "Trypan Blue - 60% Dye content" and the purity limit of ">= 80.0%" on the Specification sheet and CofA referring to the same thing? If they are, why are they different? If not, should both be considered when aiming to achieve a specific dye concentration? Additionally, if it is 60% dye, what makes up the remaining 40%? Should the significant water content in this product be taken into consideration?
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