T9784

Tricine

BioXtra, pH 4.0-6.0 (1 M in H₂O), ≥99% (titration)

• Synonym(s): N-[Tris(hydroxymethyl)methyl]glycine
• Linear Formula: (HOCH₂)₃CNHCH₂CO₂H
• CAS Number: 5704-04-1
• Molecular Weight: 179.17
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 24900603
• EC Number: 227-193-6
• Beilstein/REAXYS Number: 1937804
• MDL number: MFCD00004277

Properties

• product line: BioXtra
• Quality Segment: 400
• assay: ≥99% (titration)
• form: powder
• impurities: ≤0.005% Phosphorus (P), ≤0.1% Insoluble matter
• ign. residue: ≤0.1%
• pH: 4.0-6.0 (1 M in H₂O)
• useful pH range: 7.4-8.8
• pKa (25 °C): 8.1
• solubility: H₂O: 1 M, clear, colorless
• anion traces: chloride (Cl^-): ≤0.1%, sulfate (SO₄^2-): ≤0.05%
• cation traces: Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH₄⁺: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%
• absorption: ≤0.05 at 280 in H₂O at 1 M, ≤0.1 at 260 in H₂O at 1 M
• application(s): diagnostic assay manufacturing
• SMILES string: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• InChI key: SEQKRHFRPICQDD-UHFFFAOYSA-N

Description

General description

The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.

Application

Buffer component for separation of low molecular weight peptides.

Tricine has been used:

• in a polymerase chain reaction (PCR) buffer used in the single-cell collection[1]
• as a component of lactotroph feeding medium for culturing lactotrophs[2]
• to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer[3]

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)