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T0377

Sigma-Aldrich

Tricine

≥99% (titration)

Synonym(s):

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
5704-04-1
Molecular Weight:
179.17
Beilstein/REAXYS Number:
1937804
EC Number:
227-193-6
MDL number:
MFCD00004277
UNSPSC Code:
12161700
PubChem Substance ID:
24899884
NACRES:
NA.25

assay

≥99% (titration)

form

crystalline powder

useful pH range

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

solubility

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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General description

Tricine is the most frequently used electrophoresis buffer. It is also useful for the resuspension of cell pellets. Tricine functions as a trailing ion and aids the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems. It has a lower negative charge than glycine, which helps it migrate faster. Tricine has a high ionic strength that allows increased ion movement and less protein movement. This in turn leads to the separation of low molecular weight proteins in lower percent acrylamide gels.

Application

Tricine has been used:
  • to prevent precipitation of salts during autoclaving of Emiliania huxleyi cultures
  • as a component of buffer A for the homogenization of samples like Caenorhabditis elegans, Drosophila, and plants
  • as a component of fresh assay buffer to measure serum melatonin by radioimmunoassay

Buffer component for separation of low molecular weight peptides.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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EPPS

Assessing the applicability of Emiliania huxleyi coccolith morphology as a sea-surface salinity proxy
Fielding SR, et al.
Limnology and Oceanography, 54(5), 1475-1480 (2009)
Encyclopedia of Dairy Sciences (2011)
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Chronobiology international, 31(1), 134-143 (2013-10-19)
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The lipid droplet (LD) is a cell organelle that has been linked to human metabolic syndromes and that can be exploited for the development of biofuels. The isolation of LDs is crucial for carrying out morphological and biochemical studies of
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Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
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Protocols

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

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