D0184

2′-Deoxyuridine 5′-triphosphate sodium salt solution

100 mM in H₂O

• Synonym(s): dUTP sodium salt
• Empirical Formula (Hill Notation): C₉H₁₅N₂O₁₄P₃ · xNa⁺
• CAS Number: 94736-09-1
• Molecular Weight: 468.14 (free acid basis)
• MDL number: MFCD01630759
• UNSPSC Code: 41106305
• PubChem Substance ID: 24893218
• NACRES: NA.52

Properties

• assay: ≥99%
• form: liquid
• concentration: 100 mM in H₂O
• color: colorless
• foreign activity: DNase, RNase, none detected
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=CC(=O)NC2=O
• InChI: 1S/C9H15N2O14P3.Na.H/c12-5-3-8(11-2-1-7(13)10-9(11)14)23-6(5)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,12H,3-4H2,(H,18,19)(H,20,21)(H,10,13,14)(H2,15,16,17);;
• InChI key: FVFWWPFKJNTHIQ-UHFFFAOYSA-N

Description

General description

2′-Deoxyuridine 5′-triphosphate (dUTP) is incorporated into DNA during DNA synthesis. It is one of the main intermediate during pyrimidine nucleotide biosynthesis. It is one of the sources of uracil in DNA. Deoxyuridine 5′-triphosphate nucleotidohydrolase suppresses the incorporation of dUTP into DNA. It converts dUTP to 2′-deoxyuridine 5′-monophosphate (dUMP).

Application

2′-Deoxyuridine 5′-triphosphate sodium salt solution has been used in the mouse genomic DNA amplification by polymerase chain reaction (PCR). It is also suitable for conjunction with uracil DNA glycosylase to prevent carryover contamination in PCR. The dUTP is substituted for dTTP in the PCR reaction.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable