D5038

2′-Deoxyguanosine 5′-triphosphate trisodium salt solution

100 mM (pH 7)

• Synonym(s): dGTP-Na₃
• Empirical Formula (Hill Notation): C₁₀H₁₃N₅Na₃O₁₃P₃
• CAS Number: 93919-41-6
• Molecular Weight: 573.13
• EC Number: 300-026-5
• MDL number: MFCD00080337
• UNSPSC Code: 41106305
• PubChem Substance ID: 24893919
• NACRES: NA.52

Properties

• assay: ≥99%
• Quality Level: 200
• form: liquid
• concentration: 100 mM (pH 7)
• color: colorless
• foreign activity: DNase, RNase, none detected
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
• InChI: 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
• InChI key: IWGGLKOTEOCWQP-BIHLCPNHSA-K

Description

General description

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

Application

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

2′-Deoxyguanosine 5′-triphosphate trisodium salt solution has been used:

• in a reverse transcription-polymerase chain reaction (RT-PCR) for DNA amplification.[1]
• as a component of the PCR mix for in situ reverse transcription-polymerase chain reaction (RT-PCR) amplification of mRNA from prokaryotic and eukaryotic ribosomes[2]
• to study the mutations in the HBB gene via amplification refractory mutation system (ARMS)-PCR[3]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)