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  • A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis.

A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis.

Journal of bioscience and bioengineering (2008-08-12)
Chun Yi Liau, Chung-Sheng Lin
ABSTRAKT

A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated that our method is more sensitive and less complicated than the conventional Calcofluor white M2R staining.

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Sigma-Aldrich
Brilliant Blue R Concentrate, suitable for SDS-PAGE, methanol solution
Sigma-Aldrich
Brilliant Blue R, pure
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Brilliant Blue R, Dye content ~50 %, Technical grade
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Brilliant Blue R, 250, for microscopy
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Brilliant Blue R Staining Solution, suitable for (for immunoelectrophoresis protein staining)