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Merck

Rat Embryonic Stem Cell Transgenesis.

Methods in molecular biology (Clifton, N.J.) (2023-03-31)
Elizabeth C Bryda, Hongsheng Men, Barbara J Stone
ABSTRAKT

The availability of reliable germline competent rat embryonic stem cell (ESC) lines that can be genetically manipulated provides an important tool for generating new rat models. Here we describe the process for culturing rat ESCs, microinjecting the ESCs into rat blastocysts, and transferring the embryos to surrogate dams by either surgical or non-surgical embryo transfer techniques to produce chimeric animals with the potential to pass on the genetic modification to their offspring.

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Sigma-Aldrich
apo-Transferrin human, powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
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632 TransferTip (ES), sterile, set of 25 ea
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Accutase® solution, sterile-filtered, suitable for cell culture