This product is suitable for cell culture. Aqueous solutions of apo-transferrin should be stable at 4°C for 5–10 days. Solutions should be filter-sterilized for maximum stability. For more information, please refer to the product information sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/136/004/t1147pis.pdf
T1147
apo-Transferrin human
powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
apo-Transferrin human
Synonim(y):
Human transferrin, Siderophilin
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100 MG
1370,00 zł
500 MG
3160,00 zł
5 X 100 MG
3740,00 zł
1 G
5450,00 zł
1370,00 zł
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Pozwól nam pomócbiological source
human plasma
product line
BioReagent
assay
≥98% (agarose gel electrophoresis)
form
powder
mol wt
76-81 kDa
concentration
~25 mM
technique(s)
cell culture | mammalian: suitable
impurities
HIV and HBsAg, source material tested negative, ≤0.005% iron
solubility
H2O: 50 mg/mL
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... TF(7018)
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Ta pozycja | T0665 | T3705 | T0523 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) immunoprecipitation (IP): suitable, tissue culture: suitable | technique(s) protein quantification: suitable |
| form powder | form powder | form powder | form powder |
| biological source human plasma | biological source human plasma | biological source human | biological source mouse |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in ambient | shipped in - | shipped in - |
| solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL | solubility - | solubility H2O: 20 mg/mL |
Application
Apo-Transferrin human can be supplemented with iron or used to bind free iron present in media. It is also used to transport iron into mammalian cells.
Biochem/physiol Actions
Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human apo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Apo-transferrin can be loaded with iron prior to use or added directly to an iron containing medium.
Other Notes
Non-heme iron-transport protein.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
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Produkty
Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.
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Instructions
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Is this product sterile and can be used directly in cell culture media or does it have to be filtered first?
1 answer-
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What is the carbohydrate content of human transferrin?
1 answer-
We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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