X100RS

Triton^™ X-100 reduced

• Synonim(y): Polyoxyethylene (10) isooctylcyclohexyl ether
• Wzór liniowy: 4-(C₈H₁₇)C₆H₁₀(OCH₂CH₂)_nOH, n~10
• Numer CAS: 92046-34-9
• UNSPSC Code: 12161900
• NACRES: NA.25
• MDL number: MFCD00167048

Właściwości

• InChI: 1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3
• SMILES string: CC(C)(C)CC(C)(C)C1CCC(CC1)OCCOCCOCCOCCOCCOCCOCCO
• InChI key: QQJNBKDKLMCALZ-UHFFFAOYSA-N
• biological source: synthetic (oragnic)
• description: non-ionic
• form: viscous liquid
• mol wt: micellar average mol wt 80,000, average mol wt 625
• aggregation number: 100-155
• refractive index: n20/D 1.473 (lit.)
• CMC: 0.2-0.9
• mp: 48 -50 °C (118 - 122 °F)
• transition temp: cloud point 65 °C
• solubility: water: soluble, clear to slightly hazy, colorless
• density: 1.029 g/mL at 25 °C (lit.)
• absorption: ≤0.25 at 277 nm in H₂O at 0.5%
• HLB: 13.5
• Quality Level: 100

Opis

General description

Triton^™ X-100 reduced is a versatile nonionic surfactant featuring a hydrophilic polyethylene oxide chain, commonly employed as a laboratory detergent. Its applications span various purposes, including cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a vital component in lysis buffers. Widely utilized in biochemical and cell biology research, Triton^™ X-100 facilitates the isolation of lipid rafts, enhancing the solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, this product exhibits excellent wetting properties and emulsification enhancement.

In cell biology, Triton^™ X-100 plays a crucial role in solubilizing membrane-bound proteins, preserving their native conformation in solution. Furthermore, Triton^™ X-100 reduced, resulting from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative, has been associated with potential benefits in enzyme digestion enhancement and effects on the photoisomerization of bacteriorhodopsin. The versatility of Triton^™ X-100 in various research applications underscores its significance as a fundamental tool in biochemical and cell biology investigations.

Application

Triton^™ X-100 reduced has been used:

• in nuclear magnetic resonance (NMR) sample tube for lysing/rupturing the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles
• in cell permeabilization
• as a component in Tris/HCl to determine hydrolysis of the esters for enzyme activity assays

Features and Benefits

• Non-ionic surfactant
• Improves solubility and dispersibility of substances
• Excellent wetting properties
• Enhances emulsification
• High purity product for research applications

Preparation Note

Hydrogenated to reduce UV absorbance.

Other Notes

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Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H315,H319,H335,H411
• pcodes: P261 - P264 - P271 - P273 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Klasa składowania: 10 - Combustible liquids
• flash_point_f: 235.4 °F - closed cup
• flash_point_c: 113 °C - closed cup
• ppe: dust mask type N95 (US), Eyeshields, Gloves