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TOX6
In Vitro Toxicology Assay Kit, Sulforhodamine B based
Synonim(y):
biomass viability assay, sulforhodamine B, total biomass assay
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Wybierz wielkość
1 KIT
2400,00 zł
2400,00 zł
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Informacje o tej pozycji
NACRES:
NA.84
UNSPSC Code:
12352207
Przejdź do
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Pozwól nam pomócform
liquid
Quality Level
usage
kit sufficient for 1,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
565 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
room temp
1 of 4
Ta pozycja | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| usage kit sufficient for 1,000 tests | usage 1 kit sufficient for 2,000 tests | usage 1 kit sufficient for 1,000 tests | usage kit sufficient for 500 tests |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| storage condition dry at room temperature | storage condition dry at room temperature | storage condition dry at room temperature | storage condition dry at room temperature |
| λmax 565 nm | λmax - | λmax 540 nm | λmax 490 nm |
cytotoxicity assay substrate, colorimetric determination, 565 nM detection | cytotoxicity assay substrate, tetrazolium dye based, fluroescence determination, 600 nM detection | cytotoxicity assay substrate, colorimetric determination, 540 nM detection | cytotoxicity assay substrate, tetrazolium dye based, colorimetric determination, 490 nM detection |
General description
The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.
Application
In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.
Biochem/physiol Actions
Dye binds to cellular protein and is then solubilized in base.
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Danger
wgk
WGK 2
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
Klasa składowania
8A - Combustible corrosive hazardous materials
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P Skehan et al.
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We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
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Produkty
Testy komórkowe do proliferacji komórek (BrdU, MTT, WST1), żywotności komórek i eksperymentów cytotoksyczności do zastosowań w badaniach nad rakiem, neuronauką i komórkami macierzystymi.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Powiązane treści
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| TOX6-1KT | 04061837397950 |
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What is the Department of Transportation shipping information for this product?
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When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?
1 answer-
If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed. This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.
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Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?
1 answer-
Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension. Otherwise, you need to centrifuge, remove the media and then fix. Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003.
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