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T0665

Sigma-Aldrich

holo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥97%

Synonim(y):

Siderophilin, iron-saturated

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About This Item

Numer CAS:
11096-37-0
Numer WE:
234-318-8
Numer MDL:
MFCD00131337
Kod UNSPSC:
12352202
NACRES:
NA.75

pochodzenie biologiczne

human plasma

Poziom jakości

200

opis

Iron-saturated

linia produktu

BioReagent

Próba

≥97%

Postać

powder

metody

cell culture | mammalian: suitable

zanieczyszczenia

HIV, HBsAg and HCV, none detected (tested by FDA approved testing methods)
 endotoxin, tested

rozpuszczalność

H2O: 50 mg/mL

numer dostępu UniProt

P02787

Warunki transportu

ambient

temp. przechowywania

2-8°C

informacje o genach

human ... TF(7018)

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Zastosowanie

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.
holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.

Działania biochem./fizjol.

Provides iron to cells in a physiologically stable and safe form.

Uwaga dotycząca przygotowania

Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Komentarz do analizy

Iron content by ICP reported on a lot-specific basis.
Purity by agarose gel electrophoresis.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Co-operative signalling mechanisms required for erythroid precursor expansion in response to erythropoietin and stem cell factor
Arcasoy O and Jiang X
British Journal of Haematology, 130(1), 121-129 (2005)
Jill M Brown et al.
Nature communications, 9(1), 3849-3849 (2018-09-23)
Self-interacting chromatin domains encompass genes and their cis-regulatory elements; however, the three-dimensional form a domain takes, whether this relies on enhancer-promoter interactions, and the processes necessary to mediate the formation and maintenance of such domains, remain unclear. To examine these
Senthil Raja Jayapal et al.
Cell cycle (Georgetown, Tex.), 15(22), 3070-3081 (2016-09-23)
Cyclin A2 is an essential gene for development and in haematopoietic stem cells and therefore its functions in definitive erythropoiesis have not been investigated. We have ablated cyclin A2 in committed erythroid progenitors in vivo using erythropoietin receptor promoter-driven Cre
Pamela Sarkar et al.
Cytotherapy, 20(1), 21-28 (2017-09-18)
Clinical trials using ex vivo expansion of autologous mesenchymal stromal cells (MSCs) are in progress for several neurological diseases including multiple sclerosis (MS). Given that environment alters MSC function, we examined whether in vitro expansion, increasing donor age and progressive
Engineered mitochondrial ferritin as a magnetic resonance imaging reporter in mouse olfactory epithelium
Iordanova B, et al.
Testing, 8(8), e72720-e72720 (2013)
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