T1076
Anti-TAP antibody, Mouse monoclonal
clone 53H8, purified from hybridoma cell culture
Synonim(y):
Anti-TIP-associated protein
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About This Item
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
purified from hybridoma cell culture
rodzaj przeciwciała
primary antibodies
klon
53H8, monoclonal
Postać
buffered aqueous solution
masa cząsteczkowa
antigen ~66 kDa
reaktywność gatunkowa
human
metody
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear cell extract
izotyp
IgG1
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... NXF1(10482)
Powiązane kategorie
Opis ogólny
Monoclonal Anti-TAP (mouse IgG1 isotype) is derived from the 53H8 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from Balb/c mice immunized with TAP.
TIP-associated protein (TAP), also known as nuclear export factor (NXF1), is encoded by the gene mapped to human chromosome 11q12.3. The encoded protein is a member of multigene family of evolutionarily conserved proteins. TAP contains non-canonical RNP-type RNA binding domain, four leucine-rich repeats and an NTF2-like domain. It also contains an ubiquitin-associated domain involved in the interaction with nucleoporins.
Specyficzność
Monoclonal Anti-TAP recognizes human TAP (~66 kDa).
Immunogen
TAP (Tip Associated Protein).
Zastosowanie
Anti-TAP antibody, mouse monoclonal may be used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Działania biochem./fizjol.
TIP-associated protein (TAP) plays an important role in carrying mRNAs from the nucleus. Elevated expression of TAP or of SRp20, enhance the production of gammaretroviral Gag polyprotein. The encoded protein might facilitate the repression of two mutations caused by insertions of endogenous retroviruses into introns of cellular genes by regulating their mature transcript levels ∼2 fold.
Transporter associated with antigen processing (TAP) interacts with the RNA-binding protein Y14 protein, a participant of the mRNA-protein complex (mRNP) near the exon-exon junction of the spliced mRNAs. Formation of the TAP/p15 heterodimer is required for efficient nuclear pore complex binding and significantly enhances the ability of TAP to export mRNAs from the nucleus. In addition, TAP plays a central role in the nuclear export of (constitutive transport element) CTE containing viral mRNA. However, the recruitment of TAP to the two types of mRNA occurs by different mechanisms. TAP binds to the viral CTE directly, while the interaction with cellular mRNA is found to be indirectly.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Przechowywanie i stabilność
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Multipotent Genetic Suppression of Retrotransposon-Induced Mutations by Nxf1 through Fine-Tuning of Alternative Splicing
Concepcion D
PLoS Genetics, 5 (2009)
Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex
Kataoka N, et al.
The Embo Journal, 20(22), 6424-6433 (2001)
Menghan Mei et al.
Proceedings of the National Academy of Sciences of the United States of America, 121(22), e2314166121-e2314166121 (2024-05-20)
The nonstructural protein 1 (Nsp1) of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is a virulence factor that targets multiple cellular pathways to inhibit host gene expression and antiviral response. However, the underlying mechanisms of the various Nsp1-mediated functions and
Gammaretroviral pol sequences act in cis to direct polysome loading and NXF1/NXT-dependent protein production by gag-encoded RNA
Bartels H and Luban J
Retrovirology (2014)
De novo triplication of 11q12.3 in a patient with developmental delay and distinctive facial features
Yamamoto T
Molecular Cytogenetics, 6 (2013)
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