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T0678
Anti-Tryptophan Hydroxylase Antibody
mouse monoclonal, WH-3
Synonim(y):
Anty-TPH
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100 μL
2240,00 zł
0.2 ML
2780,00 zł
2240,00 zł
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100 μL
2240,00 zł
0.2 ML
2780,00 zł
About This Item
2240,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Rekombinowane, niezawierające konserwantów przeciwciało jest dostępne dla Twojego celu. Wypróbuj ZRB1305
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Polecane produkty
Nazwa produktu
Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, clone WH-3, ascites fluid
pochodzenie biologiczne
mouse
białko sprzężone
unconjugated
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
WH-3, monoclonal
masa cząsteczkowa
antigen 55 kDa
zawiera
15 mM sodium azide
reaktywność gatunkowa
human, monkey, rabbit, rat
metody
immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using rabbit pineal gland extract
izotyp
IgG3
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... TPH1(7166) , TPH2(121278)
rat ... Tph1(24848) , Tph2(317675)
Opis ogólny
Monoclonal Anti-Tryptophan Hydroxylase (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Tryptophan hydroxylase (TPH; L-tryptophan tetrahydropteridine:oxygen oxidoreductase; EC 1.14.16.4), the rate-limiting enzyme for the synthesis of serotonin, is a very specific enzyme found only inside serotoninergic neurons.
The tryptophan hydroxylase 1 gene (TPH1) is a 444 amino acid protein encoded by the gene spanning 29 kbp with11 exons on human chromosome 11p15.3-p14. The enzyme is expressed in the gut, spleen, thymus, pituitary and also in the pineal gland.
Specyficzność
This antibody reacts specifically with tryptophan hydroxylase.
Immunogen
recombinant rabbit tryptophan hydroxylase.
Zastosowanie
Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunoblot
- immunocytochemistry
Działania biochem./fizjol.
The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Certyfikaty analizy (CoA)
Lot/Batch Number
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Klienci oglądali również te produkty
Anthony C Ruocco et al.
Neuropsychology, 30(1), 18-27 (2015-12-29)
The tryptophan hydroxylase 1 gene (TPH1) catalyzes the formation of 5-hydroxytryptophan, a precursor to the neurotransmitter serotonin. Variations in the gene encoding this enzyme may underlie difficulties in impulse control; however, the proximate relationship between risk alleles for polymorphisms in
Brain tryptophan hydroxylase: purification of, production of antibodies to, and cellular and ultrastructural localization in serotonergic neurons of rat midbrain
Joh TH, et al.
Proceedings of the National Academy of Sciences of the USA, 72(9), 3575-3579 (1975)
Honghai Zhang et al.
Epilepsia, 57(8), 1228-1235 (2016-06-16)
The DBA/1 mouse is a relevant animal model of sudden unexpected death in epilepsy (SUDEP), as it exhibits seizure-induced respiratory arrest (S-IRA) evoked by acoustic stimulation, followed by cardiac arrhythmia and death. Defects in serotonergic neurotransmission may contribute to S-IRA.
Kadri Andre et al.
BMC psychiatry, 13, 118-118 (2013-04-20)
In major depression, one of the candidate genes possibly affecting the risk and severity of symptoms has been found to be tryptophan hydroxylase (TPH1). Variation in treatment response to antidepressive agents according to TPH1 genotype has also been found in
Peter Saetre et al.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, 153B(2), 387-396 (2009-06-16)
Serotonin (5-hydroxytryptamin; 5-HT) alternations has since long been suspected in the pathophysiology of schizophrenia. Tryptophan hydroxylase (tryptophan 5-monooxygenase; TPH) is the rate-limiting enzyme in the biosynthesis of 5-HT, and sequence variation in intron 6 of the TPH1 gene has been
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What is the Department of Transportation shipping information for this product?
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Do you have an immunohistochemistry protocol for use with Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse?
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Free floating sections (4% paraformaldehyde fixation)1. Incubate 30 minutes at room temperature in 0.3% Triton X-100, 5% normal serum.2. Incubate with the antibody diluted 1:10,000 in PBS, 0.3% Triton X-100, 3.5% normal serum and 0.05% BSA for 36-48 hours.3. Rinse 3 times for 5 minutes each in PBS.4. Add the biotinylated anti-mouse IgG3 diluted in PBS containing 0.3% Triton X-100 and 3.5% normal serum for 1 hour at room temperature.5. Rinse 3 times for 5 minutes each in PBS.6. Process with Avidin Biotin-HRP.7. Complete the reaction with a 2-10 minute incubation in PBS containing 0.05% DAB and 0.015% hydrogen peroxide (H2O2).8. Mount the sections on gel coated slides and air dry.
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Does Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, cross-react with tyrosine or phenylalanine hydroxylases?
1 answer-
This antibody, unlike some other antibodies to tryptophan hydroxylases (WH) in the market, does not cross react with tyrosine hydroxylase (TH) or with phenylalanine hydroxylase (PH). The epitope recognized by the antibody is not located in the Pterin Binding Region, which is homologous among PH, TH and WH. The specificity to WH is confirmed by the single band obtained at the 55KD region, while TH has a higher MW (60-68KD), depending on the submit) and PH has a lower MW. It has also been confirmed by collaborating experts. See Journal of Neuroscience Methods, 114(2), 205-212 (2002).
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Can Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, be used to stain mouse brain sections?
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This antibody has been used successfully on mouse brain slices in an immunohistochemical application. The IHC results from an experiment employing T0678 at 1:500 in mouse brain slice staining clearly showed the dorsal nuclear raphe (which are the serotonergic neurons).
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