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SAB4200085

Sigma-Aldrich

Monoclonal Anti-AGO2 antibody produced in rat

~1.5 mg/mL, clone 11A9, purified immunoglobulin

Synonim(y):

AGO2 Antibody - Monoclonal Anti-AGO2 antibody produced in rat, Ago2 Antibody, Anti-Argonaute 2, Anti-EIF2C2 eukaryotic translation initiation factor 2C, 2, Anti-Q10

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rat

białko sprzężone

unconjugated

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

11A9, monoclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen ~85 kDa

reaktywność gatunkowa

monkey, rat, human, mouse, bovine

opakowanie

antibody small pack of 25 μL

stężenie

~1.5 mg/mL

metody

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using HeLa cell extracts

izotyp

IgG2a

numer dostępu UniProt

Q9UKV8

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... AGO2(27161)
rat ... Ago2(59117)

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Opis ogólny

Monoclonal Anti-AGO2 (rat IgG2a isotype) is derived from the hybridoma 11A9 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a peptide corresponding to amino acids 1-15 of human AGO2. Monoclonal Anti-AGO2 recognizes human, monkey, bovine, rat and mouse AGO2. Argonaute 2 (Ago2) is encoded by the gene mapped to human chromosome 8q24.3. The encoded protein belongs to the Ago protein family. AGO2 is mainly localized on the diffuse cytoplasm and stress granules.

Zastosowanie

Monoclonal Anti-AGO2 antibody produced in rat has been used in:
  • immunofluorescent localization
  • western blot analysis
  • immunoprecipitation.

Działania biochem./fizjol.

Argonaute 2 (Ago2) plays a vital role in short interfering RNA-mediated gene silencing. The encoded protein is regulated at both the transcriptional and posttranslational level in human breast cancer cell lines. Ago2 and enhanced micro-RNA activity is involved in the tumorigenic progression of breast cancer cell lines. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC), head and neck squamous cell carcinoma patients.

Postać fizyczna

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

Store at –20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Inne uwagi

Cell lysates should not be boiled before loading onto SDS-PAGE, but only warmed to 60°C for 15 min. In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Ryan J Golden et al.
Nature, 542(7640), 197-202 (2017-01-24)
MicroRNAs (miRNAs) perform critical functions in normal physiology and disease by associating with Argonaute proteins and downregulating partially complementary messenger RNAs (mRNAs). Here we use clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) genome-wide loss-of-function screening
The Argonaute protein family
Hock J and Meister G
Genome Biology, 9 (2008)
Jessie A G L van Buggenum et al.
Scientific reports, 6, 22675-22675 (2016-03-08)
Immuno-PCR combines specific antibody-based protein detection with the sensitivity of PCR-based quantification through the use of antibody-DNA conjugates. The production of such conjugates depends on the availability of quick and efficient conjugation strategies for the two biomolecules. Here, we present
Next Generation Sequencing Analysis Reveals Segmental Patterns of microRNA Expression in Mouse Epididymal Epithelial Cells
Nixon B, et al.
PLoS ONE, 10 (2015)
Gunter Meister et al.
Molecular cell, 15(2), 185-197 (2004-07-21)
Argonaute proteins associate with small RNAs that guide mRNA degradation, translational repression, or a combination of both. The human Argonaute family has eight members, four of which (Ago1 through Ago4) are closely related and coexpressed in many cell types. To
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Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit

Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.

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