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Merck

P6757

PIPES

≥99% (titration)

Synonim(y):

1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

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25 G

318,00 zł

100 G

1210,00 zł

500 G

4170,00 zł

1 KG

7670,00 zł

318,00 zł

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C8H18N2O6S2
Numer CAS:
5625-37-6
Masa cząsteczkowa:
302.37
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24898793
EC Number:
227-057-6
Beilstein/REAXYS Number:
817713
MDL number:
MFCD00006159

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assay

≥99% (titration)

form

crystalline powder

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

InChI key

IHPYMWDTONKSCO-UHFFFAOYSA-N

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Ta pozycja
P8203P185180635
PIPES ≥99% (titration)

P6757

PIPES

PIPES BioXtra, ≥99% (titration)

P8203

PIPES

PIPES BioPerformance Certified, suitable for cell culture

P1851

PIPES

PIPES BioXtra, Molecular Biology, ≥99.5% (T)

80635

PIPES

assay

≥99% (titration)

assay

≥99% (titration)

assay

-

assay

≥99.5% (T)

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

100

form

crystalline powder

form

powder

form

crystalline powder

form

powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless, soluble

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

>300 °C (lit.)

General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al.,[1] developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Application

Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.,
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.[2]
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[3]

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

Other Notes

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation, and PIPES BioXtra (Product No. P8203) which is tested for the presence of trace metals.
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Klasa składowania

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000535246
0000541786
0000535247
0000541784
0000531688

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

The role of the buffer in the fixation of biological specimens for transmission and scanning electron microscopy
Schiff, R.I. and Gennaro, J.F., Jr.
Scanning, 2, 135-135 (1979)
Use of PIPES buffer in the fixation of plant cells for electron microscopy
Salema, R. and Brandao, I.
Journal of Submicroscopic Cytology, 9, 79-79 (1973)
A R Hardham
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 33(2), 110-118 (1985-02-01)
The study of the surface properties of zoospores and cysts of the fungus Phytophthora cinnamomi required a fixation regime that would preserve the cells adequately and not interfere with binding and detection of probes on the cell surface. When they
K Yamamoto et al.
Histochemistry, 77(3), 339-351 (1983-01-01)
Effects of NaOH-PIPES buffer used as a vehicle for aldehyde fixative on alkaline phosphatase (ALPase) activity demonstrated cyto- and biochemically were compared with those of routinely used cacodylate buffer. The reaction products showing ALPase activity demonstrated ultracytochemically were confined to
Buffers for enzymes.
J S Blanchard
Methods in enzymology, 104, 404-414 (1984-01-01)
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Powiązane treści

Buffer Solutions from Bench to Production Scale

Discover high-purity biological buffers for production scale. Ensure stability, pH control, and consistent supply with our expert manufacturing solutions.

Numer pozycji handlu globalnego

SKUNUMER GTIN
P6757-25G04061835265626
P6757-500G04061835265633
P6757-100G04061835265602
P6757-25KG04061832549910
P6757-1KG04061835265619

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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