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P8203

PIPES

BioXtra, ≥99% (titration)

PIPES
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Synonym(s):

1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

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50 g

Available to ship TODAYfromMILWAUKEE

$265.00
250 g

Available to ship TODAYfromMILWAUKEE

$1,010.00

About This Item

Empirical Formula (Hill Notation):
C8H18N2O6S2
CAS Number:
5625-37-6
Molecular Weight:
302.37
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24898917
EC Number:
227-057-6
Beilstein/REAXYS Number:
817713
MDL number:
MFCD00006159

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product line

BioXtra

Quality Level

400

assay

≥99% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900°C)

≤0.5% (as SO4)

loss

≤0.5% loss on drying, 110°C

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

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1 of 1

This Item
P6757P185180635
PIPES BioXtra, ≥99% (titration)

Sigma-Aldrich

P8203

PIPES

PIPES ≥99% (titration)

Sigma-Aldrich

P6757

PIPES

PIPES BioPerformance Certified, suitable for cell culture

Sigma-Aldrich

P1851

PIPES

PIPES BioXtra, Molecular Biology, ≥99.5% (T)

Sigma-Aldrich

80635

PIPES

product line

BioXtra

product line

-

product line

-

product line

BioXtra

assay

≥99% (titration)

assay

≥99% (titration)

assay

-

assay

≥99.5% (T)

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

100

form

powder

form

crystalline powder

form

crystalline powder

form

powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless, soluble

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al.,[1] developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Application

Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.[2],[3]
PIPES has been utilized in protein crystallization.[4],[5] The use of PIPES in the reconstitution of dissociated tubulin
α and β subunits after their resolution on immunoadsorbent gels has been described.[6] PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8.[7]
A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays.[8] PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.[9]

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

Analysis Note

Trace elemental analyses have been performed on the BioXtra PIPES; Certificate of Analysis provides lot-specific results. BioXtra PIPES is for applications which require tight control of elemental content.

Other Notes

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000545977
0000541816
0000541815
0000545977
0000521349

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Q Yu et al.
Analytical biochemistry, 253(1), 50-56 (1997-11-14)
Of the 20 well-known buffers proposed by Good, all but 3 form metal ion complexes which can result in serious interferences, particularly in protein analyses. The structural features responsible for such complex formation have been identified. Based on a mechanistic
Sambrook , J. and Russell, D.W.
Molecular Cloning: A Laboratory Manual, 7-7 (2001)
S Haviernick et al.
Journal of microscopy, 135(Pt 1), 83-88 (1984-07-01)
It is suggested that the use of Hanks' + pipes + sucrose buffers, in combination with glutaraldehyde and osmium tetroxide fixatives, represent an excellent mode of preparation of fresh and cultured peripheral blood leucocytes, not only for transmission electron microscopy
S A Moore et al.
Journal of molecular biology, 312(3), 511-523 (2001-09-21)
Human bile salt-stimulated lipase (BSSL), which is secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk, is important for the effective absorption of dietary lipids. The dependence of BSSL on bile acids
K E Loesser et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 34(11), 1477-1485 (1986-11-01)
We have modified the Lowicryl K4M low-temperature dehydration and embedding procedure for immunoelectron microscopy to provide improved ultrastructural detail and facilitate the localization of actin and tubulin in isolated rat adrenocortical cells, chick spinal cord with attached dorsal root ganglia
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Protocols

Immunofluorescence Labeling of Cells

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

Global Trade Item Number

SKUGTIN
P8203-50G04061834401186
P8203-250G04061834401179
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