NA0800

GenElute^™ HP Select Plasmid Gigaprep Kit

sufficient for 5 preparations

• Synonim(y): Szybka izolacja plazmidów, Zestaw HP gigaprep, szybki zestaw gigaprep, ultraszybka izolacja plazmidów, zestaw do ekstrakcji plazmidów, zestaw do izolacji plazmidów, zestaw do oczyszczania plazmidów, zestaw endo free gigaprep, zestaw giga bez endotoksyny, zestaw plazmidów wolnych od endotoksyny
• Kod UNSPSC: 41105501
• NACRES: NA.52

Właściwości

• zastosowanie: sufficient for 5 preparations
• charakterystyka ekologicznej alternatywy: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• metody: DNA extraction: suitable
• kategoria ekologicznej alternatywy: Aligned,
• temp. przechowywania: 15-25°C

Opis

Opis ogólny

The GenElute HP Select Plasmid Gigaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 15 mg of high copy plasmid DNA with ≤0.1 endotoxin unit/μg plasmid DNA can be recovered from E. coli cultures in 1.2-2.5 L Luria Broth (LB) medium or 600 mL-1.2 L of Terrific Broth (TB) medium in less than 2 hours after harvesting the cells. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is cleared by filtration followed by the addition of a binding solution optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured onto a silica membrane in the presence of high salts while endotoxins are prevented from adsorbing to the membrane. Contaminants are removed with three wash steps. Finally, the bound DNA is eluted in endotoxin-free water.

The DNA is ready for immediate use in applications such as restriction digest, cloning, sequencing, PCR, and transfection of endotoxin-sensitive cell lines.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Zastosowanie

GenElute^™ HP Select Plasmid Gigaprep Kit has been used to purify plasmids.[1]

High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells and gene therapy research.

Cechy i korzyści

• Up to 15 mg with endotoxin levels of ≤ 0.1 EU/μg
• Only 90 minutes from pelleted cells to purified plasmid
• Vacuum format with no ethanol precipitation
• Can purify low-, medium-, and high-copy plasmid DNA

Inne uwagi

For additional information, please see www.sigma-aldrich.com/genelutehp.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Elementy zestawu są też dostępne oddzielnie

• G3671 GenElute^™ HP Pro Gigaprep Binding Column

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02,GHS08,GHS05,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H226,H290,H302,H315,H319,H334,H336,H372
• Zwroty wskazujące środki ostrożności: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
• Organy docelowe: Central nervous system, Lungs
• Kod klasy składowania: 3 - Flammable liquids
• Temperatura zapłonu (°F): 77.0 °F - closed cup
• Temperatura zapłonu (°C): 25 °C - closed cup