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Merck

MAK092

Peroxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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Informacje o tej pozycji

NACRES:
NA.84
UNSPSC Code:
12161503

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usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric, fluorometric

storage temp.

−20°C

General description

Peroxidase (EC 1.11.1.7) is an enzyme found broadly in biological systems that utilizes hydrogen peroxide in the oxidation of various substrates.[1] The Peroxidase Activity Assay Kit provides a simple and direct procedure for measuring peroxidase activity in a variety of biological samples.

Application

Peroxidase Activity Assay Kit has been used to quantify the activity of peroxidase.[2][3]
Suitable for the determination of peroxidase activity in cell culture supernatant, serum, plasma, urine, and other biological fluids.

Biochem/physiol Actions

Peroxidase catalyzes the reaction between H2O2 and the probe, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the peroxidase activity present. One unit of peroxidase is defined as the amount of enzyme that reduces1.0 μmole of H2O2 per minute at 37°C.

Features and Benefits

Compatible with high-throughput handling systems.
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comparable product

Numer produktu
Opis
Cennik

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Klasa składowania

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 2

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
8E25K07720
8H30K07720
8E09K07720
7L11K07720
7D28K07720

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Odwiedź Bibliotekę dokumentów

Agnieszka Kiełbowicz-Matuk et al.
Frontiers in plant science, 13, 965098-965098 (2022-09-27)
The transition from vegetative growth to reproductive development is a critical developmental switch in flowering plants to ensure a successful life cycle. However, while the genes controlling flowering are well-known in model plants, they are less well-understood in crops. In
Sujitha Balakrishnan Sulochana et al.
Frontiers in bioengineering and biotechnology, 8, 585632-585632 (2020-11-17)
Scenedesmus quadricauda CASA CC202, a potent freshwater microalga is being used as a biofuel feedstock, which accumulates 2.27 fold lipid during nitrogen stress induction. Upon nitrogen starvation, S. quadricauda undergoes biochemical and metabolic changes that perturb the cell to cope
4.09 - Peroxidases
Charlene A M
Comprehensive Toxicology, 4, 169-184 (2010)
Prasad Kottayil Padmanabhan et al.
Cell death & disease, 7(10), e2406-e2406 (2016-10-14)
DDX3 is a highly conserved member of ATP-dependent DEAD-box RNA helicases with multiple functions in RNA metabolism and cellular signaling. Here, we describe a novel function for DDX3 in regulating the mitochondrial stress response in the parasitic protozoan Leishmania. We
Microscopic and molecular characterization of the prehaustorial resistance against wheat leaf rust (Puccinia triticina) in Einkorn (Triticum monococcum).
Serfling A, et al.
Frontiers in Plant Science, 7, 1668-1668 (2016)
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Protokoły

Test enzymatyczny peroksydazy (EC 1.11.1.7)

Ta procedura służy do oznaczania aktywności enzymatycznej peroksydazy przy użyciu pirogallolu jako substratu.

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. The protocol for the kit says this should be used with 96 well plates and a 96-well spectrophotometer. Can this be used for single reactions?

    1 answer

    1. This product has not been tested for single reactions. Sample peroxide values are determined by comparison to the standard curve which is prepared in the same plate. The end user would have to optimize for single reaction use. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/418/201/mak092pis-ms.pdf

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  4. If we are going to use the POD kit to evaluate the activity in broccoli juice do we need to extract and purify the enzyme? What is the lowest detection level

    1 answer

    1. If the enzyme is already in solution (that is, already in the juice), then no further sample preparation would be required. The only issue might be the intense color of the broccoli juice.

      The sample should be diluted sufficiently so the juice does not interfere with the detection (whether colorimetric or fluorometric). Also, if the broccoli juice is not clear, it is recommended to centrifuge samples before testing them. In other words, if there is insoluble particulate matter in the broccoli juice, it may need to be removed to prevent light scattering.

      The lowest detection limit for the kit is 1.0 µmol.

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