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L6669

Sigma-Aldrich

LuminoCt® qPCR ReadyMix™

For fast probe-based quantitative PCR

Synonim(y):

LuminoCt® qPCR ReadyMix™, LuminoCt®

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Wybierz wielkość

100 REACTIONS
1370,00 zł
500 REACTIONS
6000,00 zł
2000 REACTIONS
22 370,00 zł

1370,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Wszystkie zdjęcia(3)

Wybierz wielkość

Zmień widok
100 REACTIONS
1370,00 zł
500 REACTIONS
6000,00 zł
2000 REACTIONS
22 370,00 zł

About This Item

Kod UNSPSC:
41106300
NACRES:
NA.55

1370,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

Poziom jakości

200

Postać

liquid

zastosowanie

sufficient for 100 reactions
 sufficient for 2000 reactions
 sufficient for 500 reactions

Właściwości

dNTPs included
hotstart

metody

qPCR: suitable

kolor

colorless

moc wejściowa

purified DNA

przydatność

suitable for (quantitative PCR)

Zastosowanie

agriculture

kompatybilność

Bio-Rad MyiQ ( )
 Bio-Rad iCycler iQ
 Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

metoda wykrywania

probe-based

Warunki transportu

wet ice

temp. przechowywania

−20°C

Opis ogólny

Inclusion of JumpStart™ Taq antibody in the ReadyMix™ eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

  • Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms.
  • ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays.
  • Kit is not compatible with qPCR instruments that utilize glass capillary tubes.
LuminoCt® qPCR ReadyMix combines the performance enhancements of our JumpStart™ Taq antibody for hot start PCR with the convenience of an easy-to-use reaction mixture.

Zastosowanie

LuminoCt® qPCR ReadyMix™ has been used in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay to quantify the reverse transcription product or cDNA for gene expression analysis.[1][2][3] It has also been used in qPCR for quantifying the RNA extracted from rhinoviruses.[4]

Cechy i korzyści

  • Ideal for rapid, high-throughput 2-step qPCR protocols
  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced preparation time and the risk of contamination from multiple pipetting steps
  • Compatible with commonly available real-time instrument platforms for probe-based qPCR assays
  • Require small amplicons (<200 bp) for optimal results
  • Compatible with commercial primer sets, including TaqMan® assays
  • JumpStart™ Taq DNA polymerase allows room temperature set-up and the hot-start mechanism prevents primer-dimer and non-specific product formation
  • Assay results in as little as 25 minutes
  • Designed to deliver unsurpassed accuracy, precision, and sensitivity
  • Extensive design and validation of ReadyMix chemistry virtually eliminates the need to optimize assay parameters

Opakowanie

LuminoCt qPCR ReadyMix, Catalog Number L5794, contains optimized concentrations of Tris-HCl, pH 8.3, KCl, dNTPs (dATP, dCTP, dGTP, TTP), stabilizers, MgCl2 and JumpStart Taq DNA Polymerase. Provided as 100, 500 and 2000 reactions (25 μL mix in a 50 μL reaction volume).

100x ROX internal reference dye, Catalog Number R4526. Optional, for use with machines compatible with an internal reference dye, e.g., ABI and Stratagene.

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Inne uwagi

  • Commercially available 5′ nuclease assays (e.g., TaqMan® Assays) are compatible with LuminoCt ReadyMix.
  • At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are <200 bp.
  • Sigma recommends designing primers and probes with Beacon Designer software

To order primers and probes from Sigma, visit www.sigma.com/oligos

Informacje prawne

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LuminoCt is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
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Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki
  • R4526100X ROX internal reference dyeKarta charakterystyki

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Cennik

W1754

Water, PCR Reagent

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Juan Vicente Canet et al.
The Plant cell, 24(10), 4220-4235 (2012-10-16)
Salicylic acid (SA) signaling acts in defense and plant development. The only gene demonstrated to be required for the response to SA is Arabidopsis thaliana non-expresser of pathogenesis-related gene 1 (NPR1), and npr1 mutants are insensitive to SA. By focusing
Talita B Gagliardi et al.
PLoS pathogens, 18(1), e1010159-e1010159 (2022-01-08)
The clinical impact of rhinovirus C (RV-C) is well-documented; yet, the viral life cycle remains poorly defined. Thus, we characterized RV-C15 replication at the single-cell level and its impact on the human airway epithelium (HAE) using a physiologically-relevant in vitro
Laura de Diego-García et al.
Biochimica et biophysica acta. Molecular basis of disease, 1863(1), 43-51 (2016-10-22)
The Ubiquitin-Proteasome System (UPS) is essential for the regulation of the cellular proteostasis. Indeed, it has been postulated that an UPS dysregulation is the common mechanism that underlies several neurological disorders. Considering that extracellular nucleotides, through their selective P2Y2 receptor
Samer M Hussein et al.
Nature, 471(7336), 58-62 (2011-03-04)
The mechanisms underlying the low efficiency of reprogramming somatic cells into induced pluripotent stem (iPS) cells are poorly understood. There is a clear need to study whether the reprogramming process itself compromises genomic integrity and, through this, the efficiency of
Philip Owens et al.
PloS one, 8(6), e67533-e67533 (2013-07-11)
Bone Morphogenetic Proteins (BMPs) are secreted cytokines that are part of the Transforming Growth Factor β (TGFβ) superfamily. BMPs have been shown to be highly expressed in human breast cancers, and loss of BMP signaling in mammary carcinomas has been
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Produkty

Probe-based qPCR

Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

PCR Master Mix

Mieszanina wzorcowa PCR upraszcza PCR/RT-PCR dzięki składnikom takim jak polimeraza DNA, dNTP, MgCl2 i bufor, dostępnym w handlu lub DIY.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

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Protokoły

Multiplex qPCR Protocol

Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.

qPCR with Single Detection Probe

qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

SPUD Assay for Detection of Assay Inhibitors Protocol

SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.

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