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H1387
Hanks′ Balanced Salts
Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture
Synonim(y):
HBSS
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Opis ogólny
Balanced salt solution (BSS) serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It also provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. BSS when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. In addition, it also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Zastosowanie
Hanks′ Balanced Salts has been used:
- for diluting digested tissues during isolation of pancreatic blood vessels
- for washing mouse intestinal mucosal cells before lysis and sonication
- as an immersion solution to test the in vitro bioactivity of poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone
Ilość
Formulated to contain 9.7 grams of powder per liter of medium.
Rekonstytucja
Supplement with 0.35 g/L sodium bicarbonate.
Inne uwagi
The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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