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Merck

ED4SS

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

99.0-102.0% (titration)

Synonim(y):

EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate dihydrate

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Wybierz wielkość

100 G

389,00 zł

500 G

610,00 zł

1 KG

1040,00 zł

2.5 KG

2030,00 zł

389,00 zł

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Informacje o tej pozycji

Wzór liniowy:
(NaOOCCH2)2NCH2CH2N(CH2COONa)2 · 2H2O
Numer CAS:
10378-23-1
Masa cząsteczkowa:
416.20
NACRES:
NA.77
PubChem Substance ID:
24894711
EC Number:
200-573-9
Beilstein/REAXYS Number:
3861753
MDL number:
MFCD00150026
UNSPSC Code:
12352116

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Quality Level

300

assay

99.0-102.0% (titration)

form

crystalline

reaction suitability

reagent type: chelator

solubility

H2O: soluble, clear, colorless

SMILES string

O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4

InChI key

KSYNLCYTMRMCGG-UHFFFAOYSA-J

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Ta pozycja
E65110369503699
Ethylenediaminetetraacetic acid tetrasodium salt dihydrate 99.0-102.0% (titration)

Sigma-Aldrich

ED4SS

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate BioReagent, suitable for cell culture, 98.5-102.0%

Sigma-Aldrich

E6511

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate purum, ≥98.0% (KT)

Sigma-Aldrich

03695

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

Ethylenediaminetetraacetic acid tetrasodium salt hydrate BioUltra, ≥99.0% (KT)

Sigma-Aldrich

03699

Ethylenediaminetetraacetic acid tetrasodium salt hydrate

assay

99.0-102.0% (titration)

assay

98.5-102.0%

assay

≥98.0% (KT)

assay

≥99.0% (KT)

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

form

crystalline

form

powder

form

powder or crystals

form

powder

solubility

H2O: soluble, clear, colorless

solubility

H2O: 160 mg/mL

solubility

H2O: 0.1 g/mL, clear

solubility

H2O: 0.1 M at 20 °C, clear, colorless

reaction suitability

reagent type: chelator

reaction suitability

-

reaction suitability

reagent type: chelator

reaction suitability

reagent type: chelator

General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid and a metal chelating agent. It comprises four carboxylic acid and two amine groups with lone-pair electrons that can chelate calcium and several other metal ions. 

Application

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate has been used:
  • in the decalcification of femurs for the histoprocessing and staining of osteochondral tissue
  • as a component of NH4Cl lysis buffer for erythrocyte lysis and bulk characterization
  • for the isolation of crypts from the connective tissues of mice

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.
Ethylenediaminetetraacetic acid (EDTA) is used to treat patients poisoned with heavy metal ions. It functions as a chelator of the zinc ion in the active site of metalloproteases. EDTA inhibits other metal ion-dependent proteases such as calcium-dependent cysteine proteases. It might interfere with biological events which are metal ion-dependent. EDTA inhibits platelet aggregation and is the most preferred anticoagulant for platelet counts. It associates with other active agents to modulate microorganisms and biofilms, like citric acid, alcohol, antibiotics, polyhexamethylene biguanide (PHMB) quaternary ammonium compounds, and other antiseptics. EDTA is used as an anticoagulant for hematological testing, as it preserves the cellular components and morphology of blood cells. 
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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2

target_organs

Respiratory Tract

Klasa składowania

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000543936
0000534653
0000534652
0000525166
0000528497

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Palaniappan Sethu et al.
Analytical chemistry, 76(21), 6247-6253 (2004-11-02)
Leukocyte isolation from whole blood to study inflammation requires the removal of contaminating erythrocytes. Leukocytes, however, are sensitive to prolonged exposure to hyper/hypoosmotic solutions, temperature changes, mechanical manipulation, and gradient centrifugation. Even though care is taken to minimize leukocyte activation
A Chevrier et al.
Osteoarthritis and cartilage, 15(3), 316-327 (2006-09-30)
Marrow-stimulation techniques are used by surgeons to repair cartilage lesions although consistent regeneration of hyaline cartilage is rare. We have shown previously that autologous blood can be mixed with a polymer solution containing chitosan in a glycerol phosphate (GP) buffer
Giuseppe Banfi et al.
Clinical chemistry and laboratory medicine, 45(5), 565-576 (2007-05-09)
Anticoagulants are used to prevent clot formation both in vitro and in vivo. In the specific field of in vitro diagnostics, anticoagulants are commonly added to collection tubes either to maintain blood in the fluid state for hematological testing or
Geoffroy Goujon et al.
Advanced healthcare materials, 10(11), e2100059-e2100059 (2021-04-24)
Oxidative stress, which is one of the main harmful mechanisms of pathologies including ischemic stroke, contributes to both neurons and endothelial cell damages, leading to vascular lesions. Although many antioxidants are tested in preclinical studies, no treatment is currently available
Michael L Salinas et al.
Biophysical journal, 118(4), 885-897 (2019-10-22)
The increasing prevalence of adult and adolescent obesity and its associated risk of colorectal cancer reinforces the urgent need to elucidate the underlying mechanisms contributing to the promotion of colon cancer in obese individuals. Adiponectin is an adipose tissue-derived adipokine
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Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Protokoły

Procedura immunohistochemiczna

Protokół ten służy do przeprowadzenia całej procedury immunohistochemii (IHC) poprzez barwienie i wizualizację określonych antygenów w skrawkach tkanek zatopionych w parafinie.

Recycling Micro-Assay of β-NAD and β-NADH

Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

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Powiązane treści

Numer pozycji handlu globalnego

SKUNUMER GTIN
ED4SS-100G04061833609361
ED4SS-500G04061835544837
ED4SS-2.5KG04061833609385
ED4SS-1KG04061833609378

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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