Instead of optimizing the bind, wash, and release steps in conventional silica-based spin purification preps, the technology focuses on a separation by performing a single step fractionation based on the size of the biomolecules, which results in depleted impurities.
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Nazwa produktu
GenElute™-E Zestaw do oczyszczania DNA metodą pojedynczego wirowania, Reagents and consumables for clean-up of pre-purified DNA. 10, 50 or 250 purifications.
purified by
(Single-spin negative chromotography)
(Time: 3 minutes or less)
feature
Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)
Intended use (For depletion of impurities and partial fractions (<50 bp) from DNA solutions)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)
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Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
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technique(s)
DNA purification: suitable
greener alternative category
storage temp.
room temp
1 of 4
Ta pozycja | EC700 | EC800 | EC400 |
|---|---|---|---|
| technique(s) DNA purification: suitable | technique(s) - | technique(s) - | technique(s) - |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. - |
| purified by (Single-spin negative chromotography) | purified by (Single-spin negative chromotography), (Time: 3 minutes or less) | purified by (Single-spin negative chromotography), (Time: 3 minutes or less) | purified by (Single-spin negative chromotography), (Time: 30 minutes or less) |
| feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the removal of organic solvent traces from DNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.) | feature Compatible Application (Suitable for most common downstream applications, including RT-PCR, gene expression, and NGS), Intended use (For depletion of impurities from RNA solutions), Typical/expected yield (Varies by sample. Please reference user guide for more information.) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For purification of genomic DNA from cell lines), Typical/expected yield (Varies by sample. Please reference user guide for more information.) |
| greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
Application
Features and Benefits
General description
Other Notes
Preparation Note
Legal Information
Klasa składowania
10 - Combustible liquids
wgk
WGK 3
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Produkty
Zestawy do oczyszczania DNA GenElute™-E zapewniają dokładną kwantyfikację DNA i dalsze procesy enzymatyczne.
GenElute™-E DNA purification kits ensure accurate DNA quantitation and downstream enzymatic processes.
Powiązane treści
GenElute™-E Single Spin DNA and RNA purification technology enables rapid DNA and RNA extraction and purification from mammalian cells, blood, tissue, and plant tissue samples.
Major technological advances have made the production of monoclonal antibodies quicker and more efficient. There are three established platforms for antibody discovery. We offer reagents for production of monoclonal antibody libraries using each of these techniques.
Animation showing technology principle of GenElute™-E single spin negative chromatography nucleic acid purification kits
Demo video showing how to purify DNA and RNA GenElute™-E single spin nucleic acid purification kits
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Genelute-e single spin DNA and RNA purification kits use negative chromatographty to isolate nucleic acids. Can you explain how this approach simplifies workflows?
1 answer-
Helpful?
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What is the composition of the lysis buffer and clearing buffer after flowing through the resin?
1 answer-
The presence of EDTA, SDS, or excess salt can affect my PCR/ sequencing reaction. The lysis buffer information is proprietary, but we can say it is free of chaotropic salts. The resins are desalting resins so EDTA, SDS, and salts are depleted.
Helpful?
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Do we know how stable the purified DNA is through several freeze-thaw cycles?
1 answer-
This will fluctuate due to sample variability (sample collection, concentration, fragment length, sequence [GC content], storage before isolation, etc.). However, the sample is buffer exchanged into a standard storage buffer that is included in the kit (1X TE).
Helpful?
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Does the technology introduce any bias into the sample?
1 answer-
GenElute™-E does not introduce biases that some “bind-wash-elute” technologies can add because the technology separates by size, rather than by what binds and what is released.
Helpful?
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