Unfortunately, information regarding the composition of this product is not available. Endothelial cell growth supplement (ECGS) is an extract of bovine neural tissue containing growth-promoting factors for vascular endothelial cells of mammalian origin. The composition of the products has not been analyzed. According to literature, ECGS comprises acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF).
E2759
Endothelial cell growth supplement from bovine neural tissue
powder, suitable for cell culture
Synonim(y):
ECGS, Endothelial Mitogen
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Wybierz wielkość
15 MG
640,00 zł
5 X 15 MG
2030,00 zł
640,00 zł
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Informacje o tej pozycji
UNSPSC Code:
12352202
NACRES:
NA.77
Biological source:
bovine neuroblastoma
Form:
powder
Impurities:
≤20 EU/vial (LAL test)
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Pozwól nam pomócNazwa produktu
Endothelial cell growth supplement from bovine neural tissue, ECGS, suitable for cell culture
biological source
bovine neuroblastoma
form
powder
potency
2-200 μg/mL ED50/EC50
quality
endotoxin tested
packaging
pkg of 15 and 5X15 mg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤20 EU/vial (LAL test)
color
tan to brown
solubility
water: soluble (1 vial reconstituted with 1 ml 0.85% NaCl)
storage temp.
2-8°C
Quality Level
1 of 4
Ta pozycja | |||
|---|---|---|---|
| biological source bovine neuroblastoma | biological source bovine pituitary glands | biological source mouse submaxillary glands | biological source bovine pituitary glands |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| impurities ≤20 EU/vial (LAL test) | impurities ≤20 EU/vial (LAL test) | impurities ≤50 EU/vial (LAL test) | impurities endotoxin, tested |
General description
Endothelial cell growth supplement (ECGS) comprises acidic fibroblast growth factor (aFGF) and basic FGF (bFGF).
Application
Endothelial cell growth supplement from bovine neural tissue has been used as a supplement in Dulbecco′s modified Eagle′s medium supplement and F-12K media supplement for human umbilical vein endothelial cells culture.
Biochem/physiol Actions
Endothelial cell growth supplement (ECGS) improves proliferation of human saphenous vein endothelial cells (HSVEC) and human umbilical vein endothelial cells (HUVEC). It coordinates with interleukin 1 (IL-1) and mediates matrix remodelling in vascular smooth muscle cells (VSMCs). It is essential for endothelial cells proliferation and adipose-derived stem cell (ASC) differentiation. An extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGS has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.
Preparation Note
ECGS is an extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of the method of Maciag, et al., and is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.
Analysis Note
The proliferative activity of endothelial cell growth supplement (ECGS) is tested in culture using fetal bovine heart endothelial cells (ATCC CRL 1395) seeded at low density. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.
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Klasa składowania
13 - Non Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
T Maciag et al.
The Journal of biological chemistry, 257(10), 5333-5336 (1982-05-25)
Endothelial cell growth factor (ECGF) is a neural-derived mitogen capable of stimulating the proliferation of quiescent populations of human umbilical vein endothelial cells (HUV-EC). ECGF has been isolated from bovine hypothalamus in two forms: a high molecular weight from (high
T Maciag et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(11), 5674-5678 (1979-11-01)
Extracts of bovine hypothalamus were found to contain a significant level of mitogenic activity when tested in a Swiss 3T3 cell [3H]dThd incorporation assay and in a human umbilical vein endothelial cell growth assay. The mitogenic activity responsible for 3T3
Coculture of mesenchymal stem cells and endothelial cells enhances host tissue integration and epidermis maturation through AKT activation in gelatin methacryloyl hydrogel-based skin model
Zhang X, et al.
Acta Biomaterialia, 59(4), 317-326 (2017)
Human macrovascular endothelial cells: optimization of culture conditions
Terramani TT, et al.
In Vitro Cellular & Developmental Biology. Animal, 36(2), 125-132 (2000)
KML001 displays vascular disrupting properties and irinotecan combined antitumor activities in a murine tumor model
Moon CH, et al.
Testing, 8(1), e53900-e53900 (2013)
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Instructions
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Apart from FGF, is there any other growth factor in the supplement (eg. VEGF)?
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