E2759

Endothelial cell growth supplement from bovine neural tissue

powder, suitable for cell culture

• Synonim(y): ECGS, Endothelial Mitogen
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• Nazwa produktu: Endothelial cell growth supplement from bovine neural tissue, ECGS, suitable for cell culture
• pochodzenie biologiczne: bovine neuroblastoma
• Poziom jakości: 200
• Formularz: powder
• siła działania: 2-200 μg/mL ED₅₀/EC₅₀
• jakość: endotoxin tested
• opakowanie: pkg of 15 and 5X15 mg
• warunki przechowywania: avoid repeated freeze/thaw cycles
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: ≤20 EU/vial (LAL test)
• kolor: tan to brown
• rozpuszczalność: water: soluble (1 vial reconstituted with 1 ml 0.85% NaCl)
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Endothelial cell growth supplement (ECGS) comprises acidic fibroblast growth factor (aFGF) and basic FGF (bFGF).

Zastosowanie

Endothelial cell growth supplement from bovine neural tissue has been used as a supplement in Dulbecco′s modified Eagle′s medium supplement and F-12K media supplement for human umbilical vein endothelial cells culture.

Działania biochem./fizjol.

Endothelial cell growth supplement (ECGS) improves proliferation of human saphenous vein endothelial cells (HSVEC) and human umbilical vein endothelial cells (HUVEC). It coordinates with interleukin 1 (IL-1) and mediates matrix remodelling in vascular smooth muscle cells (VSMCs). It is essential for endothelial cells proliferation and adipose-derived stem cell (ASC) differentiation. An extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGS has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Uwaga dotycząca przygotowania

ECGS is an extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of the method of Maciag, et al., and is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Komentarz do analizy

The proliferative activity of endothelial cell growth supplement (ECGS) is tested in culture using fetal bovine heart endothelial cells (ATCC CRL 1395) seeded at low density. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)