The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments). This vector is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-targeting) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.
Zastosowanie
Functional Genomics/Screening/Target Validation
Inne uwagi
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
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