LB185

98062318, human blood, Lymphoblastoid

• Kod UNSPSC: 41106514

Właściwości

• product name: LB185, 98062318
• pochodzenie biologiczne: human blood
• tryb wzrostu: Suspension
• kariotyp: Not specified
• morfologia: Lymphoblastoid
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human lymphoblastoid, normal,EBV-transformed

Opis linii komórkowej

The lymphocyte cell line LB185 has been established using mononuclear cells from peripheral blood of a 56 year old normal male. T cells from the same sample have been used as controls for cellular radiosensitivity studies. Cells have been subsequently transformed with EBV. Fibroblasts have been derived from the same individual and were designated 153BR (Sigma Catalogue number. 90011808). This has been confirmed by STR profiling at ECACC. Cells are lymphhoblastoid with floating aggregates present.

Zastosowanie

Mutation studies

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 9
D5S818: 10,13
D7S820: 11
THO1: 8,9
TPOX: 8,10
vWA: 17,18

pożywka hodowlana

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Rutyna subkultury

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Inne uwagi

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