About This Item
pochodzenie biologiczne
human blood
Numer panelu HRC
2
pochodzenie etniczne
UK Caucasian
płeć
male
tryb wzrostu
Suspension
kariotyp
Not Specified
morfologia
Lymphoblastoid
metody
cell culture | mammalian: suitable
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Pochodzenie linii komórkowej
UK Caucasian Male
Opis linii komórkowej
A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.
Zastosowanie
As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.
pożywka hodowlana
RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Rutyna subkultury
Maintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.
Inne uwagi
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