RENT tsS1.1

92092515, rat endometrium, Cuboidal

• Kod UNSPSC: 41106514

Właściwości

• product name: RENT tsS1.1, 92092515
• pochodzenie biologiczne: rat endometrium
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Cuboidal
• produkty: Not specified
• receptory: Glucocorticoid
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed

Opis linii komórkowej

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43 markers. The transforming agent is a ts mutant of SV 40 T-antigen (tsA58).

pożywka hodowlana

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.

Inne uwagi

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