2fTGH cell line human

12021508, human fibrosarcoma, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: 2fTGH cell line human, 12021508
• pochodzenie biologiczne: human fibrosarcoma
• tryb wzrostu: adherent
• morfologia: Epithelial
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Opis linii komórkowej

The human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway.

Profil DNA

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

pożywka hodowlana

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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Oświadczenie o zrzeczeniu się odpowiedzialności

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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