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B9754

BIS-TRIS

≥98.0% (titration)

BIS-TRIS

Synonim(y):

2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

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Do Państwa/SKUDostępnośćCena netto
25 g
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372,00 zł
100 g
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1230,00 zł
500 g
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4950,00 zł
1 kg
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8890,00 zł
5 kg
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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C8H19NO5
Numer CAS:
Masa cząsteczkowa:
209.24
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
230-237-7
Beilstein/REAXYS Number:
2205275
MDL number:

372,00 zł


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assay

≥98.0% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable, electrophoresis: suitable

color

white

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

suitability

suitable for Western blot, suitable for column chromatography of proteins

application(s)

advanced drug delivery
diagnostic assay manufacturing
general analytical
microbiology

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

General description

Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.

Application

Bis-Tris has been used :
  • as a buffer for Electrophoresis[1]
  • as a component of transfer buffer for Western Blotting[2]

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)

Other Notes

For additional information on our range of Biochemicals, please complete this form.
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B7535RDD013B4429
technique(s)

UV/Vis spectroscopy: suitable, electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

assay

≥98.0% (titration)

assay

≥98.0% (titration)

assay

≥98.0%

assay

≥98.0%

Quality Level

400

Quality Level

400

Quality Level

100

Quality Level

400

form

crystalline powder

form

powder

form

crystalline powder

form

crystalline powder

application(s)

advanced drug delivery
diagnostic assay manufacturing
general analytical
microbiology

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

storage condition

dry at room temperature

storage condition

-

storage condition

-

storage condition

-


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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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