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Dokumenty

B9754

Sigma-Aldrich

BIS-TRIS

≥98.0% (titration)

Synonim(y):

2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

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About This Item

Wzór empiryczny (zapis Hilla):
C8H19NO5
Numer CAS:
6976-37-0
Masa cząsteczkowa:
209.24
Beilstein:
2205275
Numer WE:
230-237-7
Numer MDL:
MFCD00002853
Kod UNSPSC:
12161700
Identyfikator substancji w PubChem:
24892155
NACRES:
NA.25

Próba

≥98.0% (titration)

Postać

crystalline powder

warunki przechowywania

dry at room temperature

metody

UV/Vis spectroscopy: suitable
electrophoresis: suitable

kolor

white

przydatny zakres pH

5.8-7.2

pKa (25°C)

6.5

rozpuszczalność

H2O: 500 mg/mL, clear, colorless to very faintly yellow

przydatność

suitable for Western blot
suitable for column chromatography of proteins

Zastosowanie

diagnostic assay manufacturing
general analytical
microbiology

ciąg SMILES

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

Klucz InChI

OWMVSZAMULFTJU-UHFFFAOYSA-N

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Opis ogólny

Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.

Zastosowanie

Bis-Tris has been used :
  • as a buffer for Electrophoresis
  • as a component of transfer buffer for Western Blotting

Cechy i korzyści

  • Suitable for Biological and Biochemical Research
  • Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)

Inne uwagi

For additional information on our range of Biochemicals, please complete this form.
This page may contain text that has been machine translated.

Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H319

Zwroty wskazujące środki ostrożności

P264 - P280 - P305 + P351 + P338 - P337 + P313

Klasyfikacja zagrożeń

Eye Irrit. 2

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Gloves

Certyfikaty analizy (CoA)

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2,2-Bis(hydroxyethyl)-(iminotris)- (hydroxymethyl)-methane BIS-TRIS

Millipore

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BIS-TRIS ULTROL® Grade, ≥99% dry basis (titration), suitable where a calcium phosphate-DNA complex formation is desirable

Millipore

391335

BIS-TRIS

MOPSO ≥99% (titration)

Sigma-Aldrich

M8389

MOPSO

Tricine ≥99% (titration)

Sigma-Aldrich

T0377

Tricine

BIS-TRIS hydrochloride

SAFC

RES1164B-A7

BIS-TRIS hydrochloride

MES hydrate ≥99.5% (titration)

Sigma-Aldrich

M8250

MES hydrate

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Protokoły

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

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