This antibody is a polyclonal antibody. The immunogen was a C-terminal actin fragment (C11 peptide, Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe) that is found in all actin sequences.
A2066
Anti-α-Actin-1 (ACTA1) antibody
rabbit polyclonal
Synonim(y):
Rabbit Anti-Actin
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Wybierz wielkość
100 μL
2330,00 zł
0.2 ML
3200,00 zł
2330,00 zł
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Informacje o tej pozycji
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC (p), WB
Citations:
3426
Przejdź do
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
Anti-Actin antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 42 kDa
species reactivity
wide range, vertebrates, human, slime mold, amoeba, chicken
enhanced validation
independent
Learn more about Antibody Enhanced Validation
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 using human or animal tissue sections, indirect immunofluorescence: 1:40 using chicken fibroblasts, western blot: 1:100 using chicken gizzard extract
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)
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Powiązane kategorie
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Ta pozycja | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form ascites fluid | antibody form purified immunoglobulin |
| biological source rabbit | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| form buffered aqueous solution | form buffered aqueous solution | form - | form PBS solution |
| mol wt antigen 42 kDa | mol wt antigen 42 kDa | mol wt antigen 42 kDa | mol wt antigen ~42 kDa |
General description
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence.
The ACTA1 (actin, α 1) gene is mapped to human chromosome 1q42.13. The gene codes for actin 1, a thin filament component of skeletal muscle.
In immunoblotting, the product localizes actin in many species ranging from human skeletal muscle to amoeba. The product recognizes the 42 kDa actin band using immunoblotting techniques in human or animal tissue extracts.
The ACTA1 (actin, α 1) gene is mapped to human chromosome 1q42.13. The gene codes for actin 1, a thin filament component of skeletal muscle.
In immunoblotting, the product localizes actin in many species ranging from human skeletal muscle to amoeba. The product recognizes the 42 kDa actin band using immunoblotting techniques in human or animal tissue extracts.
Immunogen
The C-terminal actin fragment attached to Multiple Antigen Peptide (MAP) backbone.
Application
May be used to study actin structure and function and to probe binding sites of actin-binding proteins.
Rabbit anti-actin antibody can be used for western blot assays. The antibody can also be used for immunohistochemistry (1:25, using human or animal tissue sections) and indirect immunofluorescence (1:40, using chicken fibroblasts) applications.
Used to study actin structure and function and to probe binding sites of actin-binding proteins. Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates. Actin was detected at 42 kDa.
Used to study actin structure and function and to probe binding sites of actin-binding proteins. Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates. Actin was detected at 42 kDa.
Used to study actin structure and function and to probe binding sites of actin-binding proteins. Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa.
Biochem/physiol Actions
Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin. Actin protein is essential for the formation of mature platelets.
Mutations in ACTA1 (actin, α 1) is known to cause thin filament myopathy such as nemaline myopathy, congenital myopathy, actin myopathy, intranuclear rod myopathy, cap myopathy and core myopathy. Upregulation of the gene is observed in orthotopic liver transplantation without any earlier evidence of autoimmune liver disease. The encoded protein actin forms a network of filaments and is responsible for the shape of the cell. It provides cellular strength and promotes active movement of the cell.
Mutations in ACTA1 (actin, α 1) is known to cause thin filament myopathy such as nemaline myopathy, congenital myopathy, actin myopathy, intranuclear rod myopathy, cap myopathy and core myopathy. Upregulation of the gene is observed in orthotopic liver transplantation without any earlier evidence of autoimmune liver disease. The encoded protein actin forms a network of filaments and is responsible for the shape of the cell. It provides cellular strength and promotes active movement of the cell.
Anti-Actin specifically stains typical stress fibers in cultured chicken fibroblasts.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Leta S Steffen et al.
Developmental biology, 309(2), 180-192 (2007-08-07)
Titin (also called connectin) acts as a scaffold for signaling proteins in muscle and is responsible for establishing and maintaining the structure and elasticity of sarcomeres in striated muscle. Several human muscular dystrophies and cardiomyopathies have previously been linked to
The zebrafish runzel muscular dystrophy is linked to the titin gene.
Steffen L S, et al.
Developmental Biology, 309(2) (2007)
Effects of doxorubicin cancer therapy on autophagy and the ubiquitin-proteasome system in long-term cultured adult rat cardiomyocytes.
Dimitrakis P, et al.
Cell and Tissue Research, 350(2), 361-372 (2012)
Mutation-specific effects on thin filament length in thin filament myopathy.
Winter JM, et al.
Annals of Neurology, 79(6), 959-969 (2016)
Severe congenital actin related myopathy with myofibrillar myopathy features.
Selcen D.
Neuromuscular Disorders, 25(6), 488-492 (2015)
Produkty
Kontrole ładowania w aplikacji western blotting.
Loading controls in western blotting application.
Powiązane treści
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Which type of actin does Product A2066, Anti-Actin antibody produced in rabbit, recognize?
1 answer-
Helpful?
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Does Product A2066, Anti-Actin antibody produced in rabbit, react with my species?
1 answer-
The sequence use to develop this antibody is conserved across many species from amoeba to vertebrates. It should react with most samples.
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Why do I have 3 bands on my western blot when using Product A2066, Anti-Actin antibody produced in rabbit?
1 answer-
While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93, 2234-2238 (1996).
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What is the molecular weight of actin, Products A2066 and A2547?
1 answer-
Actin has a molecular weight of 42 kDa.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Are there any special considerations for immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections?
1 answer-
For FFPE sections, we recommend using enzymatic unmasking.
Helpful?
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