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RTN10

Sigma-Aldrich

GenElute Mammalian Total RNA Miniprep Kit

greener alternative

sufficient for 10 purifications

Synonim(y):

GenElute Mammalian RNA Kit, Gen Elute

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1 KIT
433,00 zł

433,00 zł


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1 KIT
433,00 zł

About This Item

Kod UNSPSC:
41105501
NACRES:
NA.52

433,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

zastosowanie

sufficient for 10 purifications

Poziom jakości

charakterystyka ekologicznej alternatywy

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

kategoria ekologicznej alternatywy

temp. przechowywania

15-25°C

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Opis ogólny

Sigma′s GenElute Mammalian Total RNA Miniprep Kit provides a simple and convenient way to isolate total RNA from mammalian cells and tissues. Protocols are provided for cells, tissues, and fibrous tissues. These protocols differ in their cell lysis and disruption conditions. Once the RNA is bound to the GenElute Binding Column, the purification procedure is the same for all starting materials. For fibrous tissues the kit must be used with proteinase K (Cat. No. P4850) to ensure effective cell disruption. The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for cesium chloride gradients, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform. Cells or tissues are lysed and homogenized in a buffer containing guanidine thiocyanate to ensure thorough
denaturation of macromolecules and inactivation of RNases. Addition of ethanol causes RNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove contaminants, RNA is eluted in 50-100 μL of Elution Solution. Up to 150 μg of total RNA can be isolated in less than 30 minutes.

If all traces of DNA contamination must be eliminated, further treatment with DNase I is recommended. DNase I digestion can be performed while the RNA is bound to the GenElute Binding Column using the On-Column DNase I Digestion Set (DNASE10 and DNASE70). Alternatively, for more stringent removal of contaminating DNA, the final RNA preparation can be treated with Amplification Grade DNase I (AMPD1).
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Zastosowanie

GenElute Mammalian Total RNA Miniprep Kit has been used to extract total RNA from tissues and cells.[1][2][3]
The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications. Note that RNA shorter than 200 nucleotides in length, such as tRNA, 5S rRNA, and 5.8S rRNA, is not recovered efficiently under the conditions used with this kit.

Cechy i korzyści

  • Purifies total RNA from up to 107 cells or 40 mg of tissue per prep
  • Yields up to 150 μg of pure, concentrated total RNA per prep
  • Recover RNA from as few as 100 cells
  • Simple and efficient–12 to 18 preps in 30 minutes
  • Faster than gravity flow anion exchange methods
  • No cumbersome steps associated with resins and magnetic slurries
  • 40% more purifications per kit than the leading supplier

Inne uwagi

The GenElute Mammalian Total RNA Purification Kit combines silica-membrane technology with a convenient spin column format for a rapid bind, wash, and elute method to prepare high quality total RNA.
For additional information, please see www.sigma-aldrich.com/totalrna.

Zasada

Samples are lysed and homogenized in guanidine thiocyanate and 2-mercaptoethanol to release RNA and inactivate RNases. Lysates are spun through a filtration column to remove cellular debris and shear DNA. The filtrate is then applied to a high capacity silica column to bind total RNA, followed by washing and elution. Up to 150 μg of total RNA can be recovered per prep in 100 μl of water. The purified RNA is ready for Northern blots (Fig. 1), RT-PCR (Fig. 2) and other common applications.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC
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Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Repr. 2 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 2 Oral - STOT SE 3

Organy docelowe

Central nervous system, Liver,Heart

Zagrożenia dodatkowe

Kod klasy składowania

3 - Flammable liquids

Temperatura zapłonu (°F)

77.0 °F - closed cup

Temperatura zapłonu (°C)

25 °C - closed cup


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Odwiedź Bibliotekę dokumentów

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Scientific Reports, 6 (2016)
DNA cloning, structural analysis, SNP detection and tissue expression
profile of the IGF1 gene in Malabari and Attappady Black goats of India
THOMAS NAICY
Journal of genetics null
Danielle M Bouchard et al.
Journal of gastrointestinal oncology, 10(5), 821-830 (2019-10-12)
Sumoylation is an important post-translational modification that involves the conjugation of the Small Ubiquitin-related Modifier (SUMO) onto target proteins. This modification is reversed through the catalytic activity of SUMO isopeptidases, known as SENPs. One of these SENPs, SENP1, was reported
Sophie Tremblay et al.
Investigative ophthalmology & visual science, 54(13), 8125-8139 (2013-11-10)
Perinatal inflammatory stress in preterm babies is associated with increased rates of severe retinopathy of prematurity (ROP) and adverse neurological dysfunction. In this study, we set out to determine the consequences of severe systemic inflammatory stress on developmental retinal vascularization
Erkko Ylösmäki et al.
Journal of virology, 87(1), 335-344 (2012-10-19)
Artificial target sequences for tissue-specific miRNAs have recently been introduced as a new means for altering the tissue tropism of viral replication. This approach can be used to improve the safety of oncolytic viruses for cancer virotherapy by restricting their

Protokoły

Poznaj podstawy blottingu Northern i Southern, wraz z protokołami i aplikacjami do przenoszenia makrocząsteczek na nośniki membranowe.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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