Hoechst 33258 solution

Name: Hoechst 33258 solution
Brand: SIGMA
Price: 1090 PLN

1 mg/mL in H₂O, ≥98.0% (HPLC)

Synonim(y):

bisBenzimide H 33258 solution

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94403-1ML

1090,00 zł

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):

C₂₅H₂₄N₆O · 3HCl

Numer CAS:

23491-45-4

Masa cząsteczkowa:

533.88

MDL number:

MFCD29074011

UNSPSC Code:

12171500

PubChem Substance ID:

329770334

NACRES:

NA.32

Assay:

≥98.0% (HPLC)

Form:

liquid

Quality level:

100

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SMILES string

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key

SMNPLAKEGAEPJD-UHFFFAOYSA-N

assay

≥98.0% (HPLC)

form

liquid

Quality Level

100

mol wt

533.88 g/mol

concentration

1 mg/mL in H₂O

color

colorless, very light green-yellow to dark green-yellow, very light yellow to dark yellow

pH

6.0-8.0 (25 °C)

solubility

DMF: soluble, H₂O: soluble

density

1.00 g/cm³

fluorescence

λ_ex 355 nm; λ_em 465 nm in TE buffer; DNA

storage temp.

2-8°C

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Ta pozycja	14530	382061	B1155
Sigma-Aldrich 94403 Hoechst 33258 solution	Sigma-Aldrich 14530 bisBenzimide H 33258	Sigma-Aldrich 382061 Bisbenzimide H 33258 Fluorochrome, Trihydrochloride	Sigma-Aldrich B1155 bisBenzimide H 33258
form liquid	form powder	form solid	form powder
assay ≥98.0% (HPLC)	assay ≥98.0% (HPLC)	assay ≥98% (HPLC)	assay ≥98% (HPLC and TLC)
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 200
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
solubility DMF: soluble, H₂O: soluble	solubility water and ethanol: 10 mg/mL, H₂O: soluble, ethanol: soluble	solubility -	solubility H₂O: 10 mg/mL, water and ethanol: 10 mg/mL
concentration 1 mg/mL in H₂O	concentration -	concentration -	concentration -

General description

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. [1]It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.[2]

Application

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA.[3] Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.[4]

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Klasa składowania

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258.

Michael R Green et al.

Cold Spring Harbor protocols, 2017(5) (2017-05-04)

Measuring the concentration of DNA using fluorometry is more sensitive than spectrophotometry, allowing the detection of nanogram quantities of DNA. In this assay, DNA preparations of known and unknown concentrations are incubated with the fluorochrome Hoechst 33258. Absorption values for

Determination of the NMR solution structure of the Hoechst 33258-d(GTGGAATTCCAC)2 complex and comparison with the X-ray crystal structure.

A Fede et al.

Structure (London, England : 1993), 1(3), 177-186 (1993-11-15)

The chromosomal stain, Hoechst 33258, binds to the minor groove of the DNA double helix and specifically recognizes a run of four A-T base pairs. Extensive biochemical and biophysical studies have been aimed at understanding the binding of the dye

Aggregation features and fluorescence of Hoechst 33258.

Natalia Busto et al.

The journal of physical chemistry. B, 119(13), 4575-4581 (2015-03-12)

The functionality of the bisbenzimide Hoechst 33258 in solution has been largely exploited in the quantification of DNA. Understanding of its behavior is essential to promote its widespread application and learning of biological processes. A detailed study of the dimerization

Multilevel regulation of the glass locus during Drosophila eye development.

Cornelia Fritsch et al.

PLoS genetics, 15(7), e1008269-e1008269 (2019-07-13)

Development of eye tissue is initiated by a conserved set of transcription factors termed retinal determination network (RDN). In the fruit fly Drosophila melanogaster, the zinc-finger transcription factor Glass acts directly downstream of the RDN to control identity of photoreceptor

BZU2/ZmMUTE controls symmetrical division of guard mother cell and specifies neighbor cell fate in maize.

Hongliang Wang et al.

PLoS genetics, 15(8), e1008377-e1008377 (2019-08-30)

Intercellular communication in adjacent cell layers determines cell fate and polarity, thus orchestrating tissue specification and differentiation. Here we use the maize stomatal apparatus as a model to investigate cell fate determination. Mutations in ZmBZU2 (bizui2, bzu2) confer a complete

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